• 关键词：污泥减量  嗜热菌  金属离子  酶活性

• 基金项目：国家自然科学基金项目(No.51078128)

• 周俊
• 1. 湖南大学环境科学与工程学院,长沙 410082;
  2. 环境生物与控制教育部重点实验室(湖南大学),长沙 410082

• 郑伟
• 1. 湖南大学环境科学与工程学院,长沙 410082;
  2. 环境生物与控制教育部重点实验室(湖南大学),长沙 410082

• 李小明
• 1. 湖南大学环境科学与工程学院,长沙 410082;
  2. 广西大学环境学院,南宁 530004;
  3. 环境生物与控制教育部重点实验室(湖南大学),长沙 410082

• 杨麒
• 1. 湖南大学环境科学与工程学院,长沙 410082;
  2. 环境生物与控制教育部重点实验室(湖南大学),长沙 410082

• 陈伟
• 1. 湖南大学环境科学与工程学院,长沙 410082;
  2. 环境生物与控制教育部重点实验室(湖南大学),长沙 410082

• 罗琨
• 1. 湖南大学环境科学与工程学院,长沙 410082;
  2. 环境生物与控制教育部重点实验室(湖南大学),长沙 410082

• 摘要：利用从土壤中采集分离出来的嗜热菌进行嗜热酶溶解(S-TE)污泥稳定化处理,同时添加4种金属离子(K⁺、Ca²⁺、Mg²⁺、Fe²⁺),考察各金属离子对剩余污泥水解效果的影响.实验结果表明,在一定浓度范围内,4种金属离子都能强化污泥酶促反应,反应5 h左右时蛋白酶和淀粉酶活力达到最大值.反应过程中,200 μmol · L^-1 K⁺对蛋白酶活性的促进作用最为明显,5 h时蛋白酶活力最高可达2.20 U · mL^-1,相比空白样提高了48.6%,Ca²⁺、Mg²⁺和Fe²⁺的最佳投加浓度分别为200、100和100 μmol · L^-1,相应浓度下蛋白酶活力分别可达到1.91、1.92和2.14 U · mL^-1.K⁺对淀粉酶活的促进作用也最显著,且实验范围内离子浓度越高作用越明显,400 μmol · L^-1 K⁺情况下淀粉酶活力最大可达3.64 U · mL^-1,Ca²⁺在所取浓度范围内均促进淀粉酶活性,最大可提高35.0%,添加100 μmol · L^-1 Mg²⁺后淀粉酶活最大值可达2.02 U · mL^-1,200 μmol · L^-1 Fe²⁺能使淀粉酶活从2.42 U · mL^-1(空白样)提高到3.22 U · mL^-1.SCOD在酶促反应前期增加明显,后期增长趋于平缓,添加200 μmol · L^-1 Mg²⁺后SCOD最高可达到4590.73 mg · L^-1,相比空白样提高了12.6%.氨氮和溶解性磷酸盐受金属离子的促进作用不明显.

• Abstract：A thermophilic bacteria isolated from garden soil was used in sludge solubilization tests (S-TE tests).Four kinds of metal ions were added to identify their influence on sludge hydrolysis during the processes.The results showed that the four metal ions could facilitate the hydrolysis process to some extent within certain concentration ranges.The highest activity of protease and amylase were observed at 5 h after hydrolysis.The strongest activation (2.20 U · mL^-1) of protease could be obtained at 5 h with a K⁺ concentration of 200 μmol · L^-1, which was 48.6% higher than the control test.The optimal concentration of Ca²⁺, Mg²⁺ and Fe²⁺ for sludge hydrolysis were 200, 100 and 100 μmol · L^-1, and the corresponding protease activity reached 1.91, 1.92 and 2.14 U · mL^-1, respectively.K⁺ also had the strongest affect on amylase hydrolysis; the highest amylase activity reached 3.64 U · mL^-1 when the concentration of K⁺ was 400 μmol · L^-1.Ca²⁺ could improve amylase activity within the concentration ranges chosen in this study,and the activity was enhanced by a maximum of 35.0% compared with the control test.The amylase activity reached 2.02 U · mL^-1 at a Mg²⁺ concentration of 100 μmol · L^-1, and it could be increased from 2.42 U · mL^-1 to 3.22 U · mL^-1 with a Fe²⁺ concentration of 200 μmol · L^-1 compared with the control.The concentration of SCOD increased significantly in the initial stage of enzymatic hydrolysis, and then tended to increase slightly in the later stage.The maximum concentration of SCOD reached 4590.73 mg · L^-1 at a Mg²⁺ concentration of 200 μmol · L^-1, which was 12.6% higher than the control.The investigation also revealed that the metal ions had no obvious effects on the changes of NH⁺₄-N and soluble phosphate.

• Key words：reduction of sludge  thermophilic bacteria  metal ion  enzyme activity

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