研究报告
孙国胜,贾晓珊.多菌种生物膜内各菌种生物量的动力学推定及验证[J].环境科学学报,2011,31(8):1627-1632
多菌种生物膜内各菌种生物量的动力学推定及验证
- Development and validation of a kinetic model for the biomass of different bacterial strains in a multi-strain biofilm
- 基金项目：国家重大科技专项(No.2008ZX07211-003)
- 作者
- 单位
- 孙国胜
- 1. 中山大学环境科学与工程学院,广州 510006;
2. 广东粤港供水有限公司,深圳 518021
- 贾晓珊
- 中山大学环境科学与工程学院,广州 510006
- 摘要：以Monod模型为基准,推导多菌种生物膜内各菌种生物量的推定模型.以东深供水原水生物预处理工程为研究对象,对生物填料进行静态、批量实验,推算生物膜内亚硝化菌(AOB)和硝化菌(NOB)在生物池内的沿程分布规律及其基质限制条件;进行生物膜内AOB和NOB的培养计数实验及反应器系统出水模拟,验证生物量推定结果.结果表明:多菌种生物膜内AOB和NOB生物量的动力学推定,方法简单、可行;生物膜内AOB和NOB的活性生物量沿池长均呈两头低中间高的特殊分布;膜内AOB和NOB的活性生物量分别占相应总生物量的68.2%~74.2% 和 25.0%~29.9%;以这些活性的AOB和NOB生物量推定结果进行反应器系统出水模拟效果相当理想.
- Abstract：A series of static experiments were done to study the biomass distribution along the length of bio\\contact oxidation tanks and the effect of limiting substrates on ammonia oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB) in Dong-shen raw water bio-pretreatment tanks.Based on the Monod model,a kinetic model of different strains of biomass was set up and analyzed.In order to test the biomass kinetic model, cultivation experiments were conducted on the bio-film to detect and count AOBs and NOBs, and outflow simulation experiments were also undertaken.The study showed that the methods of the kinetics model based on the AOB and NOB biomass in the biofilm were simple and feasible.The AOB and NOB biomass in the biofilm was low at both ends and high in the middle of the biotank.The active biomass of AOB and NOB accounted for 68.2%~74.2% and 25.0%~29.9% of the total biomass,respectively.The biomass kinetic model was used to infer the outflow of the biofilm reactor with excellent simulation efficiency.
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