• 关键词：MBR  CAS  16S rDNA  PCR-DGGE  微生物群落结构  克隆测序

• 基金项目：天津市自然科学基金重点项目(No. 07 JCZD JC02100)

• 胡怡杉
• 天津大学环境科学与工程学院,天津 300072

• 孙宝盛
• 天津大学环境科学与工程学院,天津 300072

• 王盛勇
• 1. 天津大学环境科学与工程学院,天津 300072;
  2. 中钢武汉安全环保研究院,武汉 430081

• 摘要：采用聚合酶链式-变性梯度凝胶电泳(PCR-DGGE)技术,研究了膜生物反应器(MBR)和传统活性污泥工艺(CAS)反应器中微生物在贫营养条件下的总细菌群落结构.结果表明,在培养过程中,污泥的微生物种群经历了一个比较明显的变化过程,且以CAS污泥微生物种群的变化更为明显,演替过程中既有原始优势种群的消亡,又有新的优势种群的增强,故优势种群的功能地位处于动态变化中.两种污泥的来源、工艺进水水质和运行条件的不同造成了活性污泥中菌群结构的差异.两种污泥中主要存在的优势菌种大部分为未经培养菌种(uncultured bacterium)、弓形杆菌属(Arcobacter)、假单胞菌属(Pseudomonas)、芽孢杆菌属(Bacillus)、γ-变形菌纲(gamma proteobacterium)细菌等,此外,亦存在一些病原性微生物,如Arcobacter属、Serratia 属和Klebsiella属等.

• Abstract：Total microbial community structure in membrane bio-reactor (MBR) and conventional activated sludge (CAS) processes operated under oligotrophic environment was investigated using PCR-denaturing gradient gel electrophoresis (PCR-DGGE). Investigation results show that microbial population of the two types of sludge had significant changes during cultivation process, especially for the sludge in CAS process. In the evolvement of microbial population, the original dominant species was replaced by new species gradually and the original dominant species was even died out from the system. The state of the dominant populations was dynamically changing. Different sludge sources, influent water quality and operating conditions of the two systems lead to the differences of microbial community structure in activated sludge. The main dominant bacteria in the two types of sludge are uncultured bacterium, e.g. Arcobacter, Pseudomonas, Bacillus and gamma proteobacterium bacteria. Moreover, there also exist some pathogenic microorganisms, such as Arcobacter, Serratia and Klebsiella species.

• Key words：membrane bio-reactor (MBR)  conventional activated sludge (CAS)  16S rDNA  PCR-denaturing gradient gel electrophoresis (PCR-DGGE)  microbial community structure  clone and sequence

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