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研究报告

Biodegradation of 1,1-dichloroethene using benzene as aerobic cometabolism substrate

基金项目：国家自然科学基金项目(No.40102027,50578151);北京市自然科学基金项目(No.8052017);北京市产学研项目(No.51900265005);国家科技重大专项(No.2009ZX07207-008,2009ZX07419-002,2009ZX07207-001);中央高校基本科研业务专项基金(No.2011YXL016)

摘要：为更好地去除地下水中常见的混合污染物苯与1,1-二氯乙烯(1,1-DCE),从用苯与1,1-DCE驯化的好氧共代谢活性污泥中筛选获得6株菌株,并利用16S rDNA技术鉴定菌株属性,通过研究6株菌株的降解特性获得优势菌株,并进一步探讨优势菌株的最佳生长因素.结果表明,筛选的6株菌株中,4株属于产碱杆科,另外2株属于放线菌纲,且6株菌株均能降解苯和1,1-DCE.菌株DB-Ⅱ为优势菌株,且在苯初始浓度90 mg·L^-1、1,1-DCE初始浓度0.60 mg·L^-1、菌悬液吸光度0.400、pH=7、温度为25 ℃时的降解效果最好.菌株DB-Ⅱ在不同温度下对苯和1,1-DCE的降解符合一级反应动力学,且在10~25 ℃内,菌株对苯和1,1-DCE生物降解的活化能分别为41.97 kJ·mol^-1和53.10 kJ·mol^-1.

Abstract：In order to find a more effective way to remove commonly mixed contaminants in groundwater, six cometabolic strains were screened from aerobic cometabolism sludge acclimatized to benzene and 1,1-dichloroethene (1,1-DCE). The strains' properties were identified by 16S rDNA and their degradation characteristics were investigated to identify the dominant strain. It was demonstrated that four strains were Alcaligenaceae and the other two were Actinobacteria. The six cometabolism strains could degrade benzene and 1,1-DCE and DB-Ⅱ was the dominant strain. The optimum reaction conditions for strain DB-Ⅱ were as follows: initial concentration of benzene and 1,1-DCE up to 90 mg·L^-1 and 0.60 mg·L^-1,respectively, OD₆₀₀ of 0.400, a neutral pH of 7 and 25 ℃. The removals of benzene and 1,1-DCE by strain DB-Ⅱ fit first-order reaction kinetics at different temperatures. The biodegradation activation energies of benzene and 1,1-DCE from 10 to 25 ℃ were 41.97 kJ·mol^-1 and 53.10 kJ·mol^-1, respectively.

Key words：1,1-dichloroethene benzene biodegradation cometabolism kinetics activation energy