• 关键词：共代谢降解  4-氯苯酚  苯酚  动力学  恶臭假单胞菌

• 基金项目：国家自然科学基金项目(No.51009050)

• 李晶
• 1. 河海大学环境学院,浅水湖泊综合治理与资源开发教育部重点实验室,南京 210098;
  2. 泰州市环境科学研究所,泰州 225300

• 饶婷
• 河海大学环境学院,浅水湖泊综合治理与资源开发教育部重点实验室,南京 210098

• 李巍
• 河海大学环境学院,浅水湖泊综合治理与资源开发教育部重点实验室,南京 210098

• 李轶
• 河海大学环境学院,浅水湖泊综合治理与资源开发教育部重点实验室,南京 210098

• 摘要：对恶臭假单胞菌(Pseudomonas putida LY1)共代谢降解苯酚和4-氯苯酚(4-CP)系统进行了降解实验和动力学研究.结果表明,恶臭假单胞菌可以有效地降解苯酚,苯酚浓度为50 mg·L^-1时细菌生长速度最快,4-氯苯酚浓度的增加会对细菌产生一定的抑制作用.同时,用改进的Haldane方程模拟恶臭假单胞菌LY1单独降解苯酚的过程,并根据实验数据利用MATLAB软件求出了方程参数值为:最大比降解速率R_m=0.113 mg·mg^-1·h^-1,饱和常数K_s=3.5 mg·L^-1,抑制常数K_p=126.6 mg·L^-1.在此基础上修正改进的Haldane方程用以模拟恶臭假单胞菌共代谢降解苯酚和4-氯苯酚,利用MATLAB软件并结合6组共代谢降解实验(PC1~PC2,PC5~PC8)数据解出未知参数K₁,n₁,K₂,n₂,发现参数改进的Haldane方程经过修正后能很好地模拟恶臭假单胞菌共代谢降解苯酚和4-氯苯酚的过程.该模型为污水中难降解有机物的生物降解提供了理论参考.

• Abstract：Degradation of phenol and 4-chlorophenol by Pseudomonas putida LY1 was studied. It was found that phenol can be effectively degraded by the bacteria with an optimum phenol concentration at 50 mg·L^-1. Inhibition of the bacteria increased with the 4-chlorophenol concentration. A modified Haldane equation was used to study the biodegradation of phenol by Pseudomonas putida LY1. The equation parameters calculated using MATLAB software were as follows: R_m=0.113 mg·mg^-1·h^-1, K_s =3.5 mg·L^-1 and K_p =126.6 mg·L^-1. The modified Haldane equation was also used to simulate the cometabolic biodegradation of phenol and 4-chlorophenol by Pseudomonas putida LY1. The cometabolic degradation model can simulate the process of cometabolic degradation very well. The model provides a theoretical and technical reference for the biodegradation of chlorinated refractory organic pollutants in wastewater treatment plants.

• Key words：cometabolic degradation  4-chlorophenol  phenol  kinetics  Pseudomonas putida LY1

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