• 关键词：.喜树碱  普通小球藻  毒性试验  生物农药

• 基金项目：浙江省重大项目(No.2008C02007-4);浙江农林大学研究生科研创新基金(No.2112009004)

• 廖文莉
• 浙江农林大学,林业与生物技术学院,临安 311300

• 马建义
• 浙江农林大学,林业与生物技术学院,临安 311300

• 童森淼
• 浙江农林大学,林业与生物技术学院,临安 311300

• 陈安良
• 浙江农林大学,林业与生物技术学院,临安 311300

• 马良进
• 浙江农林大学,林业与生物技术学院,临安 311300

• 张立钦
• 浙江农林大学,林业与生物技术学院,临安 311300

• 摘要：以普通小球藻(Chlorella vulgaris)为试验材料,连续10 d测定了新型生物农药喜树碱3种不同的制剂对普通小球藻的毒性.结果表明:0.2%喜树碱乳油对普通小球藻毒性最高(EC₅₀值在0.0368~0.2547 mg·L^-1),其中第3天的EC₅₀值最小为0.0368 mg·L^-1;0.5%喜树碱钠盐的毒性较低(EC₅₀值在0.6659~2.3104 mg·L^-1 ),比较稳定,其中7 d的EC₅₀值最大; 2~6 d的0.5%喜树碱钾盐的毒性逐渐增加, 第6天的EC₅₀值最低为0.4684 mg·L^-1, 第9天后由0.8024 mg·L^-1急剧增加至5.8423 mg·L^-1.比较而言,喜树碱钾盐毒性和钠盐毒性较低,乳油制剂毒性最高.

• Abstract：In order to determine the toxicity of three camptothecin formulations on Chlorella vulgaris, 10- day tests were employed and data were determined every day. The results showed that 0.2% camptothecin EC had the highest toxicity to C. vulgaris in the 10d with EC₅₀ values between 0.0368 mg·L^-1 and 0.2547 mg·L^-1, with the lowest value on the 3^rd day. The toxicity of 0.5% camptothecin Na⁺ SL was low, with their EC₅₀ value between 0.6659 mg·L^-1 and 2.3104 mg·L^-1. The toxicity of 0.5% camptothecin K⁺ SL increased gradually from the 2^nd to the 8^th days, with the lowest EC₅₀ value of 0.4684 mg·L^-1 on the 6^th day. EC₅₀ increased rapidly from 0.8024 mg·L^-1 to 5.8423 mg·L^-1on the 9^th day. The results also showed that 0.5% camptothecin K⁺ and Na⁺ SL had the lowest toxicity, while the 0.2% camptothecin EC had the highest toxicity.

• Key words：camptothecin  Chlorella vulgaris   toxic effects  natural pesticide

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