研究报告
刘晓丽,汪奇,贾林芝,周忠良.壬基酚对斑马鱼精巢组织及性激素合成酶基因表达的影响[J].环境科学学报,2011,31(11):2523-2529
壬基酚对斑马鱼精巢组织及性激素合成酶基因表达的影响
- Alterations in testicular histology and the mRNAs of enzymes responsible for sex steroid synthesis in the zebrafish Danio rerio exposed to nonyphenol
- 基金项目：上海自然科学基金(No.08ZR1407300)
- 作者
- 单位
- 刘晓丽
- 华东师范大学生命科学学院, 上海 200062
- 汪奇
- 华东师范大学生命科学学院, 上海 200062
- 贾林芝
- 华东师范大学生命科学学院, 上海 200062
- 周忠良
- 华东师范大学生命科学学院, 上海 200062
- 摘要：壬基酚(NP)是广泛存在于水体中的环境内分泌干扰物, 会影响鱼类的生殖和发育. 为了解NP影响鱼类精巢发育的分子机制, 将成年雄性斑马鱼(Danio rerio)暴露于不同浓度(0、125、250、500 μg·L-1)NP下21 d, 用常规组织学方法研究试验鱼精巢组织结构的变化,并用荧光定量PCR (QRT-PCR)方法检测试验鱼精巢性激素合成酶及雌、雄激素受体(ERα、AR)基因的表达. 结果表明,暴露于250 μg·L-1NP的斑马鱼精巢内精子及精小囊的数目减少, 非细胞区域增加; 500 μg·L-1 NP组斑马鱼生精小管内精子凝集于管腔中央. 250 μg·L-1NP可导致精巢CYP17 mRNA的表达量显著下调, 125 μg·L-1NP可导致CYP11B mRNA的表达量显著下调, 并呈现出明显的负剂量-效应关系. 但是,NP对精巢AR mRNA的表达无明显影响, 精巢中CYP19A mRNA及ERα mRNA的表达与NP暴露浓度之间呈正剂量-效应关系, 125 μg·L-1NP即可显著上调CYP19A mRNA及ERα mRNA的表达. NP可通过抑制精巢中雄激素合成相关酶基因的表达影响精巢发育, 同时可诱导精巢内源雌激素合成和雌激素受体的表达, 提高雌激素效应.
- Abstract：Nonylphenol (NP), an aquatic endocrine disruptor, is known to be capable of adversely affecting fish development and reproduction. In order to elucidate the molecular mechanisms for the harmful effects of NP on testicular development in fish, the male zebrafish Danio rerio were exposed to 0, 125, 250 and 500 μg·L-1 NP for 21 days. Histological alterations in the testis and the mRNAs of enzymes responsible for sex steroid synthesis, estrogen receptor α (ERα) and androgen receptor (AR) were subsequently investigated. Histologically, the fish treated with 250 μg·L-1 NP exhibited reduced numbers of both spermatocyst and spermatozoan and an enlargement of acellular zone. In the fish exposed to 500 μg·L-1 NP, sperms were found to be congregated in the lumen of seminiferous tubules. Downregulation of CYP17 mRNA was observed in the fish treated with 250 μg·L-1 NP, while 125 μg·L-1 NP markedly downregulated the expression of CYP11 gene in the testis in a concentration-response manner. NP was found to have no effects on testicular AR mRNA. However, a concentration-response relationship was observed between NP exposure and CYP19A mRNA or ERα mRNA, with 125 μg·L-1 NP significantly increasing both CYP19A and ERα mRNAs in the testis. These results suggested that NP can render its adverse effects on testicular development through altering the expression of genes encoding enzymes involved in the synthesis of male sex hormone. Additionally, NP appeared to be capable of enhancing estrogenic effects of female sex hormone by inducing the synthesis of female sex hormone and the expression of ERα gene.
- Key words：nonylphenol zebrafish (Danio rerio) testis CYP17 CYP11B CYP19A
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