• 关键词：污水生物处理  氧化亚氮  碳源  硝化  反硝化  DGGE

• 基金项目：国家自然科学基金(No.21007032);山东大学自主创新基金(No.2009JQ009);国家水专项(No. 2009ZX07210-009-04)

• 赵聪聪
• 山东大学环境科学与工程学院,济南 250100

• 张建
• 山东大学环境科学与工程学院,济南 250100

• 胡振
• 山东大学环境科学与工程学院,济南 250100

• 谢慧君
• 山东大学环境研究院,济南 250100

• 张婷婷
• 山东大学环境科学与工程学院,济南 250100

• 李一冉
• 山东大学环境科学与工程学院,济南 250100

• 摘要：碳源是污水生物处理过程中N₂O释放的重要影响因素.本试验以不同碳源(葡萄糖、乙酸钠和淀粉)条件下的SBR反应器为研究对象,分析了碳源类型对于N₂O释放的影响作用.结果发现,N₂O释放主要发生在好氧段,且释放总量受碳源影响,以乙酸钠为碳源的反应器产生的N₂O最多,释放速率最大,其次为葡萄糖,淀粉最少,N₂O转化率分别为7.80%,5.43%,2.59%.OUR试验结合PCR-DGGE分析,以乙酸钠为碳源时氨氧化菌优势种群发生了变化,测序结果表明其为Nitrosomonas 属细菌,因此N₂O释放的差异是不同碳源条件下AOB菌群不同及其反硝化能力的不同所造成的.

• Abstract：Carbon source is an important factor affecting the emission of nitrous oxide (N₂O) in biological wastewater treatment processes. In this study, the effect of carbon source on N₂O emission was evaluated in three sequencing batch reactors (SBRs) acclimated under different carbon sources (i.e. glucose, acetate, and starch). Our results showed that most of the N₂O emission occurred during the aerobic phase. Carbon source had an important effect on N₂O emission. The highest amount and rate of N₂O emission was observed in the SBR acclimated under acetate followed in order by glucose and starch. The conversion rate of N₂O was determined to be 7.80%,5.43%,and 2.59%,respectively. According to results from OUR measurements and PCR-DGGE analyses, the dominating population of AOB community (e.g. Nitrosomonas in acetate SBR) was found to considerably change with varying carbon sources. This suggested that the effect of carbon source on N₂O emission was most likely attributed to the difference in AOB community and the associated denitrification capability.

• Key words：biological nitrogen removal  nitrous oxide  carbon source  nitrification  denitrification  DGGE

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