• 关键词：厌氧氨氧化  反应器  低速搅动床  固定床  FISH

• 基金项目：国家自然科学基金资助项目(No. 10972049)

• 高彦宁
• 1. 大连理工大学化工与环境生命学部化工机械学院,大连 116024;
  2. 国立熊本大学工学部环境工学学科,熊本 860-8555,日本

• 刘志军
• 大连理工大学化工与环境生命学部化工机械学院,大连 116024

• 古川宪治
• 国立熊本大学工学部环境工学学科,熊本 860-8555,日本

• 摘要：采用低速机械搅拌装置,以无纺布作为固定床的填料,把搅拌床与固定床的优点有机结合起来,设计一种复合式厌氧氨氧化反应器.在反应器中,生物滞留量相对较高,微生物的性状由接种时的碎絮状很快转变成颗粒状,短期内反应器系统的脱氮性能迅速提高,启动时间大为缩短.仅用38 d,反应器中总氮去除速率稳定增长至1.9 kg·m^-3·d^-1.随后的17 d,总氮去除速率倍增至3.8 kg·m^-3·d^-1.反应器运行180 d,最大的总氮去除速率达6.6 kg·m^-3·d^-1,比厌氧氨氧化菌活性维持在0.32 kg·kg^-1·d^-1左右.微生物颗粒粒径分布及SEM观测结果表明,低速机械搅拌可很好地促进微生物颗粒的生长.FISH分析显示,微生物颗粒中Anammox菌的含量约为70%.

• Abstract：A hybrid reactor for anaerobic ammonia oxidation (anammox) process was developed which combined a fixed-bed using non-woven fabrics as biomass carrier with a slow speed agitated bed. The performance of higher biomass retention was obtained in the reactor, and the anammox microorganism in a loose flocculating structure before inoculation formed rapidly into granules. The nitrogen removal ability of the reactor increased obviously in the short term, and the start-up time of the reactor shortened greatly. Only in 38 days, the total nitrogen removal rate (TNRR) reached 1.9 kg·m^-3·d^-1 and then doubled to 3.8 kg·m^-3·d^-1 within 17 days. During 180 operating days, a maximum TNRR of 6.6 kg·m^-3·d^-1 was obtained, and the specific anammox activity (SAA) maintained basically in 0.32 kg·kg^-1·d^-1. The testing results of size distribution and SEM observation confirmed that anammox sludge granulation process can be greatly enhanced in the mechanical stirred reactor at a lower rotating speed. The anammox bacteria content in the microorganism sludge was about 70%, determined by situ hybridization (FISH) analysis method.

• Key words：Anammox  reactor  slow speed agitated bed  fixed bed  FISH

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