研究论文
林娜,郭楚玲,郭延萍,柯林,党志,谭凤仪.红树林湿地中好氧-厌氧反硝化菌脱氮特性及其种群结构分析[J].环境科学学报,2012,32(1):173-181
红树林湿地中好氧-厌氧反硝化菌脱氮特性及其种群结构分析
- Community structure and denitrification ability of aerobic and anaerobic denitrifying bacteria from mangrove wetland
- 基金项目：中央高校基本科研业务费专项资金资助(No.2009ZM0034);国家自然科学基金(No.41101291)
- 作者
- 单位
- 林娜
- 华南理工大学环境科学与工程学院, 工业聚集区污染控制与生态修复教育部重点实验室, 广州 510006
- 郭楚玲
- 华南理工大学环境科学与工程学院, 工业聚集区污染控制与生态修复教育部重点实验室, 广州 510006
- 郭延萍
- 华南理工大学环境科学与工程学院, 工业聚集区污染控制与生态修复教育部重点实验室, 广州 510006
- 柯林
- 华南理工大学环境科学与工程学院, 工业聚集区污染控制与生态修复教育部重点实验室, 广州 510006
- 党志
- 华南理工大学环境科学与工程学院, 工业聚集区污染控制与生态修复教育部重点实验室, 广州 510006
- 谭凤仪
- 香港城市大学生化学院, 香港
- 摘要：随着沿岸水体氮素富营养化的加剧,生物脱氮作用越来越受重视,位于海陆交界的湿地红树林生态系统作为一个自然脱氮体系备受关注.本研究以典型亚热带湿地红树林(香港Mai Po)作为对象,结合传统的富集筛选和分子生物学方法——建立nosZ基因克隆文库和RFLP分析技术对红树林沉积物中反硝化细菌脱氮能力、种群结构和丰度进行研究.从Mai Po红树林沉积物中共筛选到12株好氧反硝化菌和8株厌氧反硝化菌,其中好氧反硝化菌包括Pseudomonas(4株)、Comamonas(2株)和Acinetobacter(2株)等7个菌属,厌氧反硝化菌属于Pseudomonas、Agrobacterium和Uncultured Betaproteobacteria bacterium(2株)等7个菌属.筛选到的好氧和厌氧反硝化菌均具有较高的脱氮能力,大部分在2 d内NO3--N去除率达到98%以上.建立Mai Po红树林湿地反硝化细菌的nosZ基因克隆文库的结果表明,反硝化细菌的50个克隆子中有26个克隆子分属于11个未知类群,其余克隆子属于Pennisetum类群(26%),β-proteobacterium类群(10%),Entandrophragma类群(4%),Pseudomonas类群(4%)和denitrifying bacterium类群(4%).可见Mai Po红树林中反硝化细菌具有很高的生物多样性.
- Abstract：Mangrove wetlands dominate the coastline of tropical and subtropical regions. With the expansion of human activity along riverine and coastal shorelines, mangroves are subject to anthropogenic inputs, which introduce additional nitrogen to mangrove ecosystems. In the present study, both traditional cultivation and molecular method (clone library and RFLP) were used to investigate the community structure and abilities of denitrifying microorganism from Mai Po mangrove sediment. Twenty aerobic and anaerobic denitrifying bacteria strains were obtained and most of them had the abilities to remove 98% NO3--N in 2 days. The aerobic denitrifying bacterial strains belonged to the genera of Pseudomonas, Comamonas and Acinetobacter. The anaerobic denitrifying bacterial strains were Pseudomonas, Agrobacterium, Uncultured Betaproteobacteria bacterium. To investigate the diversity of denitrifying bacteria in the mangrove sediments, nosZ gene clone library were constructed. 26 out of 50 clones belonged to 11 unknown groups, while the others were the Pennisetum(26%), the β-proteobacterium(10%), the Entandrophragma(4%), the Pseudomonas(4%) and the denitrifying bacterium(4%). This indicated that Mai Po mangrove had high degree of taxonomic diversity of denitrifiers.
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