• 关键词：鼠李糖脂  表面性能  胶束性质  增溶  三氯生

• 基金项目：国家科技重大专项 (No. 2008ZX07211-005)

• 林晖
• 华南理工大学环境科学与工程学院,工业聚集区污染控制与生态修复教育部重点实验室,广州 510006

• 胡勇有
• 1. 华南理工大学环境科学与工程学院,工业聚集区污染控制与生态修复教育部重点实验室,广州 510006;
  2. 华南理工大学制浆造纸工程国家重点实验室,广州 510640

• 张潇予
• 华南理工大学环境科学与工程学院,工业聚集区污染控制与生态修复教育部重点实验室,广州 510006

• 郭艳平
• 华南理工大学环境科学与工程学院,工业聚集区污染控制与生态修复教育部重点实验室,广州 510006

• 李文杰
• 华南理工大学环境科学与工程学院,工业聚集区污染控制与生态修复教育部重点实验室,广州 510006

• 冯聪
• 华南理工大学环境科学与工程学院,工业聚集区污染控制与生态修复教育部重点实验室,广州 510006

• 摘要：测定了铜绿假单胞杆菌诱变株MIG-N146产鼠李糖脂粗提物(RL-CE)及经柱层析分离纯化得到的单糖脂(RL-F1)和双糖脂(RL-F2)的表面性能及胶束性质,并通过溶解平衡实验考察了这3种组分对极性疏水物质三氯生的增溶作用.结果表明,RL-CE、RL-F1、RL-F2的临界胶束浓度CMC值分别为60.31、43.82 和115.00 mg·L^-1,对应的表面张力γ_CMC分别为29.1、31.6、28.8 mN·m^-1.具双亲水基团的RL-F2有最低的表面饱和吸附量及最大单分子平均横截面积,其溶液有分子聚合体、胶束、囊泡及多层囊泡等多种缔合体,且大粒径聚集体的丰度高.RL-F1中的平均胶束粒径最小且丰度较低.RL-CE的缔合体粒径基本呈双峰分散趋势,主要为粒径较大的胶束.当鼠李糖脂浓度高于其CMC值时,能通过胶束溶解显著提高三氯生的水相表观溶解度.RL-CE、RL-F1、RL-F2对三氯生的摩尔增溶比(MSR)分别为0.2858、0.2548、0.3505,能有效增溶三氯生.胶束内部空间越大,对三氯生的增溶能力越强,对三氯生的增溶能力排序为RL-F2 > RL-CE > RL-F1.

• Abstract：Mono-rhamnolipid (RL-F1) and di-rhamnolipid (RL-F2) were separated and purified through column chromatography of rhamnolipid crude extracts (RL-CE) produced by Pseudomonas aeruginosa mutant MIG-N146. The surface and micellization properties of three rhamnolipidic fractions were tested and their solubilizations of polar hydrophobic triclosan were investigated by solution equilibrium experiments. Results showed that the critical micelle concentration (CMC) of RL-CE, RL-F1 and RL-F2 were 60.31 mg·L^-1, 43.82 mg·L^-1 and 115.00 mg·L^-1, while the surface tension (γ_CMC) were 29.1 mN·m^-1, 31.6 mN·m^-1 and 28.8 mN·m^-1, respectively. RL-F2, with the structure of two hydrophilic groups, had the lowest saturated surface excess and the largest mean surface area per molecule. The solution of RL-F2 included several complexes of molecular polymers, micelles, vesicles and multi-vesicles, with larger aggregates showing higher abundance. Among the three rhamnolipidic fractions, RL-F1 had the smallest average micelle grainsize and lower abundance. Grainsize of the complexes in RL-CE displayed a bi-peak dispersion trend, which mainly contributed to large micelles. When the concentration of rhamnolipid was above the CMC, aqueous apparent solubility of triclosan was significantly enhanced by micelle solubilization. The molar solubilization ratio (MSR) of RL-CE, RL-F1 and RL-F2 to triclosan could be determined as 0.2858, 0.2548 and 0.3505, respectively, indicating that the solubility of triclosan was effectively enhanced. The larger interior volume of micelles, the stronger solubilization of triclosan by rhamnolipid displayed. The order of solubilization of triclosan was RL-F2 > RL-CE > RL-F1.

• Key words：rhamnolipid  surface activity  micellization  solubilization  triclosan

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