• 关键词：三维荧光  平行因子法  反应器  核黄素  蛋白质  辅酶NADH

• 基金项目：国家自然科学基金(No.50878203,21007001);"十一五"科技支撑计划(No.2008BADC4B18)

• 李卫华
• 1. 中国科学技术大学化学系, 合肥 230026;
  2. 安徽建筑工业学院 环境与能源工程学院, 合肥 230022

• 盛国平
• 中国科学技术大学化学系, 合肥 230026

• 陆锐
• 中国科学技术大学化学系, 合肥 230026

• 俞汉青
• 中国科学技术大学化学系, 合肥 230026

• 汤利华
• 安徽建筑工业学院 环境与能源工程学院, 合肥 230022

• 黄显怀
• 安徽建筑工业学院 环境与能源工程学院, 合肥 230022

• 摘要：为建立反应器运行状态的荧光光谱快速监测方法,应用三维荧光光谱表征了高温厌氧反应器的出水,采用平行因子分析方法分析后得到3种荧光组分——蛋白质、辅酶NADH和核黄素,并获得它们的激发发射光谱图及相对浓度.结果表明,当温度从50 ℃上升到60 ℃的过程中,3种荧光物质的相对浓度均呈现相应的变化,它们与反应器的运行参数呈现明显相关性.进一步研究表明,核黄素是高温厌氧产氢反应中的特有荧光物质,采用标准加入法和二阶校正方法可以测定其实际浓度,出水中核黄素浓度变化范围为0.04~0.13 mg·L^-1.该研究为厌氧产氢反应器的运行监控提供了理论基础.

• Abstract：A rapid method was established to characterize the operating status of bioreactor by fluorescence spectra. In this study, the effluent from a thermophilic hydrogen-producing upflow anaerobic sludge blanket (UASB) reactor was characterized by the three-dimensional excitation emission matrix (EEM) fluorescence spectra. With the parallel factor analysis (PARAFAC) method, the EEM fluorescence spectra and the relative concentration of three components, i.e., proteins, NADH and riboflavin, were acquired. Results showed that the relative concentration of the three fluorophores varied correspondingly when the temperature was increased from 50℃ to 60℃, and they were correlated to the operating parameters of the UASB reactor. Furthermore, riboflavin was a specific fluorophore in the effluents from the thermophilic UASB reactor. Using standard addition method and second-order calibration approach, the concentrations of the riboflavin were obtained, which varied from 0.04 to 0.13 mg·L^-1.This study provided a theoretical basis for monitoring anaerobic reactors.

• Key words：EEM fluorescence spectra  parallel factor analysis(PARAFAC)  reactor  riboflavin  protein  NADH

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