研究报告
郑朝成,周立祥.污泥中一株产耐高温蛋白酶菌株的分离、鉴定及酶学特性[J].环境科学学报,2012,32(3):577-583
污泥中一株产耐高温蛋白酶菌株的分离、鉴定及酶学特性
- Isolation and characterization of protease-producing Bacillus sp. TC16 and its enzyme features
- 基金项目：国家自然科学基金(No.21177060); 国家高技术研究发展计划(No.2009AA06Z317)
- 作者
- 单位
- 郑朝成
- 南京农业大学资源与环境科学学院环境工程系,南京 210095
- 周立祥
- 南京农业大学资源与环境科学学院环境工程系,南京 210095
- 摘要：蛋白酶是水解蛋白质肽键的一类酶的总称,它可将蛋白质分解、转化为氨基酸,从而被动植物体直接吸收利用.基于此,以江苏省无锡市太湖新城污水处理厂的污泥为材料,通过观察水解圈大小和蛋白酶活性测定等方法,从中筛选出一株高产蛋白酶菌株TC16.同时,参照《伯杰氏细菌鉴定手册》和《常见细菌鉴定手册》,根据其形态学特征、生理生化特性,并结合16S rDNA序列的比对分析结果发现,该菌属于芽孢杆菌属菌株.对其酶学性质的研究发现,从该污泥中分离得到的菌株TC16分泌的胞外蛋白水解酶的活性最高可达621 U·mL-1.研究表明,与其他芽孢杆菌属菌株相比,TC16菌株分泌的蛋白酶具有较高的酶活性,且具有较宽范围的pH和温度耐受性.
- Abstract：Proteases are a kind of enzymes that can hydrolyze protein peptide bond and decompose proteins into amino acids which could then be absorbed and utilized directly by animals and plants. The method of clear zone in combination with protease activity detection was used to test the protease activity of a screened sewage sludge-borne strain TC16. The isolate was classified into Bacillus according to its morphology, physiological and biochemical features, and 16S rDNA sequence comparison and analysis. In addition, extracellular protease activity was also detected in the cultural supernatant of the strain Bacillus sp. TC16 was identified as a mesophily bacterium with a wide range of pH and temperature tolerance as well as high enzyme activity comparing with other strains.
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