• 关键词：非光合固碳微生物  固碳  电子供体  析因实验  DGGE图谱

• 基金项目：国家科技支撑重大项目(No.2010BAK69B13);上海市重大科技攻关项目(No.10dz1200903)

• 武满满
• 同济大学环境科学与工程学院污染控制与资源化研究国家重点实验室,上海 200092

• 孙佩哲
• 同济大学环境科学与工程学院污染控制与资源化研究国家重点实验室,上海 200092

• 胡佳俊
• 同济大学环境科学与工程学院污染控制与资源化研究国家重点实验室,上海 200092

• 王磊
• 同济大学环境科学与工程学院污染控制与资源化研究国家重点实验室,上海 200092

• 李军
• 同济大学环境科学与工程学院污染控制与资源化研究国家重点实验室,上海 200092

• 廖元琨
• 同济大学环境科学与工程学院污染控制与资源化研究国家重点实验室,上海 200092

• 魏恺
• 同济大学环境科学与工程学院污染控制与资源化研究国家重点实验室,上海 200092

• 摘要：通过混合电子供体析因实验,定量描述了Na₂S、NaNO₂、Na₂S₂O₃ 3种无机物的用量和比例与非光合微生物固碳量之间的关系,并分析了3种电子供体之间的两两交互作用.最后,通过16S rDNA V3区DGGE图谱分析,研究了不同混合电子供体对固碳微生物群落结构的影响.结果表明,电子供体析因实验的模型具有较好的拟合度,能对混合电子供体的固碳效率进行较好地预测.电子供体交互作用分析结果表明,NaNO₂ 与Na₂S对菌群固碳能力有互相抑制的作用,Na₂S与Na₂S₂O₃则起到相互促进的作用.NaNO₂单独作用时,对微生物的固碳作用有较大的促进作用,而Na₂S₂O₃的加入则产生了一定的抑制效应.DGGE图谱得到了不同电子供体组合对固碳微生物群落结构的影响,不同电子供体的加入导致预培养条件下优势菌的消失,并出现了新的优势菌,这些新的优势菌有的具有固碳优势,有的则会与固碳优势菌产生竞争.DGGE图谱可从分子水平上解释电子供体及其交互组合对微生物固碳效率的影响.

• Abstract：Carbon dioxide is one of the main greenhouse gases, but it is also the most abundant carbon source, so that its recycle and reuse has become an important issue. In our experiment, a factorial design was used to demonstrate the interaction effects of different electron donors on CO₂ fixation by a non-photosynthetic microbial community under aerobic conditions, and PCR-DGGE technology was applied to analyze the changes of microbial community structure during CO₂ fixing with different electron donors. The results showed that the factorial design models were of high quality to predict the CO₂ fixation efficiency of the non-photosynthetic microbial community. Na₂S and Na₂S₂O₃ could each enhance its CO₂ fixation efficiency, and the positive effect was reinforced when both were present. NaNO₂ as added electron donor could significantly enhance CO₂ fixation, but this effect was competitively inhibited when Na₂S₂O₃ or Na₂S was added to the medium. The DGGE patterns revealed significant differences among microbial communities cultivated with various electron donors, demonstrating changes of CO₂ fixation efficiency at the molecular level. Nitrobacteria, which were predominant when pre-incubated under conditions in which NH⁺₄ was sole electron donor, disappeared in general when other electron donors were added, but many other strains which grow well in the medium with different electron donors appeared. Some of them might fix CO₂ efficiently, but some of them might compete with the bacteria which can fix CO₂.

• Key words：non-photosynthetic microbial community  CO₂ fixation  electron donors  factorial design  DGGE pattern

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