研究论文
李红岩,杨敏,张昱,高峰.失稳硝化膜生物反应器的性能恢复研究[J].环境科学学报,2012,32(2):276-281
失稳硝化膜生物反应器的性能恢复研究
- Study on the recovery of a failed nitrifying MBR system
- 基金项目：国家高技术研究发展计划(No. 2007AA06A414);国家自然科学基金项目(No. 50808171)
- 作者
- 单位
- 李红岩
- 中国科学院生态环境研究中心环境水质学国家重点实验室,北京 100085
- 杨敏
- 中国科学院生态环境研究中心环境水质学国家重点实验室,北京 100085
- 张昱
- 中国科学院生态环境研究中心环境水质学国家重点实验室,北京 100085
- 高峰
- 中国建筑设计研究院机电院给排水所,北京 100044
- 摘要：对高水力负荷条件下丧失硝化功能的膜生物反应器进行恢复实验,将水力停留时间(HRT)从原来的5 h延长到正常条件下可以实现完全硝化的10 h后,在进水NH4+-N浓度为500 mg·L-1的条件下反应器可去除99%的NH4+-N,但NO2--N出现严重积累,在60 d 的实验过程中NO2--N平均出水浓度为425 mg·L-1.荧光原位杂交分析结果表明,氨氧化菌(AOBs)在总菌中的比例与恢复实验前没有变化,分别为12.9%(恢复实验期)和9.75%(恢复实验前),但氨氧化杆菌(Nitrosomonas)在AOBs中的比例从80％降低到40%;亚硝酸盐氧化细菌(NOBs)在总菌中的比例下降一半(从5.64%下降至2.84%),并以慢生型的Nitrospira为主.高NO2-含量和高胞外物浓度(497.1 mg·L-1)可能是导致亚硝酸盐氧化功能难以恢复的主要原因.
- Abstract：In this study, we investigated the recovery process of a nitrifying membrane bio-reactor (MBR) system, which failed to achieve complete nitrification under the stress of high hydraulic loading (HRT) (5 h).When the HRT was increased from 5 h to 10 h (the condition permitting complete nitrification previously) at an influent NH4+-N concentration of 500 mg·L-1, an NH4+-N removal of 99% was achieved.However, serious NO2--N accumulation occurred, and the average NO2--N concentration in the effluent was 425 mg·L-1 during the experimental period (60 days).Fluorescence in situ hybridization (FISH) analysis showed that while the percentage of ammonia-oxidizing bacteria (AOBs) in total bacteria did not change (12.9% in this study compared to 9.75% at the HRT of 5 h in previous study), the percentage of fast-growing Nitrosomonas sp.in AOBs decreased from 80% to 40%.The percentage of nitrite-oxidizing bacteria (NOBs) was only half of that at 5 h HRT (from 5.64% to 2.84%), and the slow-growing Nitrospira sp.remained as the dominant nitrite oxidizing population.It is possibly the high NO2- and extracellular (497·1 mg·L-1) concentrations in the reactor that made the nitrate oxidizing function difficult to recover.
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