欢迎访问《环境科学学报》编辑部网站！

快速检索			高级检索

研究报告

Screen and analysis of degradation ability for crude oil degradation strains from South China Sea

基金项目：国家自然科学基金(No.41076108,40906062);国家海洋局近岸海域生态环境重点实验室开放基金(No.201008);海洋生物遗传资源重点实验室开放基金(No.HY201106);涉外海洋科学研究项目;山东省科技发展计划项目(No.2011GGF01074);海洋一所基本科研业务费专项资金(No.2010G23)

摘要：以南海10个采样点采集到的样品为研究材料,以石油降解率为筛选依据,初筛获得52株石油降解菌,从中进一步筛选出6株对石油烃有降解能力的细菌,通过16S rRNA序列分析对筛选得到的6株菌进行初步鉴定,并使用GC-MS内标法测定降解产物,对降解菌的降解特性进行进一步研究.结果表明,采用重量法筛选出来的6株细菌对石油的降解率为20%~55%.与Genbank中的16S rRNA基因序列BLAST对比结果显示,所筛选出的6株菌株中,3株菌株属于芽孢杆菌属(Bacillus),2株菌株属于假交替单胞菌属(Pseudoalteromonas),1株属于交替单胞菌属(Alteromonas).降解特性分析表明,所筛选6株菌的烷烃降解率均在40%以上,多环芳烃降解率均在70%以上,其中,菌株B08 500 m-3对石油中总烷烃和总芳香烃的降解效果较好,降解率分别为75%和87%.

Abstract：The study was performed to test the petroleum biodegradation ability of 52 bacterial strains using gravimetric method. All of these bacteria were collected from 10 samples of water from the South China Sea. Chemical analysis of biodegradation was accomplished with GC-MS, screening out six strains that had strong de-emulsification and degradation activity. The six bacterial strains was identified using 16S rRNA sequencing. The experimental results show that after screening test using the gravimetric method, the biodegradation rates of six bacterial strains were determined between 20% and 55%. The six selected strains of bacteria were compared to genus-species in Genbank through contrast of the BLAST. Three strains belonged to Bacillus, one strain to Pseudoalteromonas, and one strain to Alteromonas. The six strains showed high degradation rates, up to 40% with alkanes and 70% with polycyclic aromatic hydrocarbon (PAHs). The bacterial strain B08 500 m-3 had the highest rates with 75% reduction of alkanes and 87% degradation of PAHs.