• 关键词：白地霉  扩大培养  固定化  最佳絮凝条件  硬度  矿化度

• 基金项目：山西省研究生创新项目(No.20090508)

• 郭彦涵
• 山西大学生命科学学院,太原 030006

• 李佳月
• 山西大学生命科学学院,太原 030006

• 李保珍
• 山西大学生命科学学院,太原 030006

• 张晓峰
• 山西省煤炭地质水文勘查研究院,太原 030006

• 王兰
• 山西大学生命科学学院,太原 030006

• 摘要：为了使白地霉固定化小球处理太原市地下岩溶水达到最佳絮凝效果,分别研究了白地霉菌体的最佳扩大培养条件.同时, 分别选用海藻酸钠(SA)、聚乙烯醇-海藻酸钠(PVA-SA)及聚乙烯醇-海藻酸钠-明胶 (PVA-SA-明胶)作为固定化剂,固定化白地霉菌体,进行絮凝性能分析.最后,确定了固定化小球处理岩溶水的最佳搅拌时间及投加量,并与六水合氯化铝、自制三元复合吸附剂及壳聚糖的5%冰醋酸溶液的絮凝效果进行了比较.结果表明,将白地霉种子培养基按3%的接种量接入白地霉扩大培养基3中进行扩大培养,可在第2 d获取活性较好的大量白地霉菌体进行固定化.处理岩溶水时应选用PVA-SA-明胶固定化的白地霉小球,并在其投加量为4 g·L^-1、搅拌时间为60 min时,对岩溶水矿化度、硬度的最大絮凝率分别为96.88%、50.00%.经与化学混凝剂絮凝性能对比发现,微生物絮凝剂具有操作简便、无二次污染、絮凝效果较好的优点.

• Abstract：The preparation and activity assay of the immobilization of Geotrichum candidum were investigated and the capability of its chemical adsorbent was analyzed with the application of Microbial flocculants in treating Karst water with the high hardness and salinity. The results showed that G. candidum culture medium (No.3) was proved to be the best medium for expand training, and G. candidum with plenty of good activity for immobilization was obtained on the second day. The three kinds of ways with sodium-alginate, polyvinyl alcohol mixed with sodium alginate and alginate-gelatin-polyvinyl to immobilize G. candidum were used in this study and the optimum flocculation conditions to treat Karst water, by analyzing the flocculation capability of G. candidum immobilization pellets, were 4 g·L^-1 PVA-SA-gelatin immobilized pellets and stirring for 60 min. The biggest flocculation rate on salinity and hardness were 96.88% and 50.00%. These results indicate the microbial flocculants will have the characteristics and advantages of easy operation, no secondary pollution, and better flocculation effects compared to the chemical adsorbents.

• Key words：Geotrichum candidum  expand training  immobilization  optimum flocculation conditions  hardness  salinity

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