• 关键词：二氧化硫  心脏功能  ATP酶  信号转导通路

• 基金项目：国家自然科学基金资助项目(No.21107064)

• 张全喜
• 山西大学环境医学与毒理学研究所、山西大学环境科学与工程研究中心,太原 030006

• 孟紫强
• 山西大学环境医学与毒理学研究所、山西大学环境科学与工程研究中心,太原 030006

• 摘要：为了探讨二氧化硫(SO₂)对心脏功能影响的机制,分别用10、300和1000 μmol·L^-1的SO₂灌流大鼠离体心脏后,测定心脏组织中一氧化氮(NO)、超氧化物歧化酶(SOD)、丙二醛(MDA)和谷胱甘肽(GSH)含量以及心脏灌流液中肌酸激酶(CK)和乳酸脱氢酶(LDH)活性.结果表明, 10、300和1000 μmol·L^-1的SO₂均可使心脏组织中NO和MDA含量以及心脏灌流液中CK和LDH活性明显增加,而使SOD和GSH含量明显减少.较高浓度(300和1000 μmol·L^-1)的SO₂可使心脏组织中Na⁺K⁺-ATP酶和Ca²⁺Mg²⁺-ATP酶活性明显下降.结论:SO₂对大鼠离体心脏功能影响的作用机制与SO₂对心脏组织的氧化损伤作用、心肌细胞膜Na⁺K⁺-ATP酶和Ca²⁺Mg²⁺-ATP酶活性降低及NO-cGMP信号转导通路有关.

• Abstract：To investigate the mechanism of the effects of sulfur dioxide (SO₂) on heart function, isolated rat hearts were perfused by 10, 300 and 1000 μmol·L^-1 SO₂, respectively. Then the contents of nitric oxide (NO), superoxide dismutase (SOD), reduced glutathione (GSH), malondialdehyde (MDA) in the hearts and the activities of lactate creatine kinase (CK) and dehydrogenase (LDH) in the perfusate were measured. The results showed that, at 10, 300 and 1000 μmol·L^-1,SO₂ significantly increased the contents of NO and MDA in the hearts and the activities of CK and LDH in the perfusate. But the contents of SOD and GSH in the hearts were significantly decreased. At high concentrations (300 and 1000 μmol·L^-1), SO₂ decreased the activities of Na⁺K⁺-ATPase and Ca²⁺Mg²⁺-ATPase in the hearts. These results suggested that the mechanism of the effects of SO₂ on heart function might be related to the oxidative damage effect of SO₂ on heart tissues, the decreased activities of Na⁺K⁺-ATPase and Ca²⁺Mg²⁺-ATPase, and the NO-cGMP signal transduction pathway.

• Key words：sulfur dioxide  heart function  ATPase  signal transduction pathway

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