研究报告
孙静,丁德馨,胡南,李广悦,王永东.诱变绳状青霉吸附铀的行为研究[J].环境科学学报,2012,32(10):2384-2393
诱变绳状青霉吸附铀的行为研究
- Characteristics for biosorption of uranium from aqueous solutions by mutated Penicillium funiculosum
- 基金项目：国家自然科学基金项目(No.50774047);湖南省科技厅重点项目(No.2010GK2025);湖南省教育厅重点项目(No.10A103)
- 作者
- 单位
- 孙静
- 南华大学铀矿冶生物技术国防重点学科实验室, 衡阳 421001
- 丁德馨
- 南华大学铀矿冶生物技术国防重点学科实验室, 衡阳 421001
- 胡南
- 南华大学铀矿冶生物技术国防重点学科实验室, 衡阳 421001
- 李广悦
- 南华大学铀矿冶生物技术国防重点学科实验室, 衡阳 421001
- 王永东
- 南华大学铀矿冶生物技术国防重点学科实验室, 衡阳 421001
- 摘要：先后采用盐酸羟胺和紫外光辐射,对绳状青霉(Penicillium funiculosum)进行诱变,并用红外光谱分析了诱变绳状青霉细胞表面官能团的变化,采用静态吸附试验研究了铀溶液的pH、温度、初始铀浓度、吸附时间、菌体投加量等因素对其吸附铀的影响.通过对动力学模型、等温吸附模型和热力学方程进行拟合,研究了诱变绳状青霉吸附铀的行为.采用扫描电镜、能谱仪和红外光谱仪分析了吸附前后诱变绳状青霉细胞表面的形貌、化学组成和官能团结构的变化,进而探讨了吸附过程可能涉及的反应机理.结果表明,诱变绳状青霉在pH=6时吸附铀的效果最好,8h即达到吸附平衡,最大吸附量为200mg·g-1,对铀的吸附为细胞表面的缔合—OH、—CHO、不饱和键、—NH2、—PO43-等与铀的配位络合反应.
- Abstract：Penicillin funiculosum was mutated by hydroxyl-ammonium chloride and ultraviolet radiation in succession,and the alterations of functional groups on the surfaces of the dead cells of the mutated Penicillin funiculosum were analyzed by FTIR.Static experiments were conducted on the effects of pH,temperature,initial concentration of uranium,contact time and dosage on the biosorption of uranium from aqueous solutions by the mutated Penicillin funiculosum.The biosorption performances of the mutated Penicillin funiculosum for uranium were investigated by correlating the experimental data with the existed kinetic models,isothermal models and thermodynamic equations.The surface morphology,chemical composition,functional groups and the possible mechanism were analyzed for the mutated Penicillin funiculosum before and after biosorption treatment using SEM,EDS and FTIR.The results showed that the removal of uranium by the mutated Penicillin funiculosum reached the maximum at pH 6.0 on the experimental conditions.,The biosorption came to equilibrium in 8 h,and the maximum adsorption capacity was estimated to be 200 mg·g-1 by using the Langmuir adsorption model.The adsorption was in the form of complexation reaction between the uranium and the organic groups such as —OH,—CHO,unsaturated bonds,—NH2,and PO43- on the surfaces of the cells.
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