• 关键词：蚯蚓  超微结构  阿苯达唑  精细胞  生态毒理

• 基金项目：国家自然科学基金项目(No.31172091);河北省自然科学基金(No.C2012204074)

• 高玉红
• 1. 中国农业大学资源环境学院, 北京 100094;
  2. 河北农业大学动物科技学院, 保定 071001

• 贾鎏辉
• 河北科技工程技术学校, 保定 071001

• 吴占军
• 河北省农林科学院粮油作物研究所, 石家庄 050031

• 孙振钧
• 中国农业大学资源环境学院, 北京 100094

• 摘要：选择不影响蚯蚓生长的染毒剂量(100mg·kg^-1)和半数致死剂量(600mg·kg^-1),检测兽药阿苯达唑对蚯蚓精子发生的显微和超显微结构的影响.结果表明,光镜下观察贮精囊时,100mg·kg^-1的阿苯达唑对精细胞和精子发育没有影响,而600mg·kg^-1时,精细胞发育明显受阻,且未见成熟的精子.电镜下观察贮精囊时,100mg·kg^-1的阿苯达唑损伤了精子发生的超微结构,表现为初期精细胞核萎缩变形,微管排列紊乱,精细胞膜扩张;发育后期精子细胞的线粒体嵴和基质消失;成熟精子的糖原颗粒异常分布于核周围,鞭毛的电子密度和糖原颗粒增加.而阿苯达唑剂量为600mg·kg^-1时,精细胞严重损伤,核膜消失,甚至染色质消失.可见,精子发生的超微结构变化可以为早期土壤污染预警提供理论基础.

• Abstract：The study was to investigate the effect of veterinary medicine albendazole on microstructure and ultrastructure during spermiogenesis in earthworms.Earthworms were exposed to albendazole at 0 mg·kg^-1 (control),100 mg·kg^-1 (no effect on earthworms growth) and 600 mg·kg^-1 (LD₅₀).Under optical microscopy,no changes in seminal vesicle were observed at 100 mg·kg^-1 compared to the control,while the spermatid development was inhibited at 600 mg·kg^-1,with no mature sperms being found.Electron microscopy revealed that ultrastructure during spermiogenesis changed at 100 mg·kg^-1.In the early stage of spermiogenesis,the deformed nuclear was observed at 100 mg·kg^-1,microtubule's arrangement was abnormal and outer membrane of spermatid was enlarged.In the late stage,the cristae and matrix content in mitochondria disappeared at 100 mg·kg^-1,and dense glycogen granules were located abnormally around nuclear.We also found out that electron dense and glycogen granules in flagellum piece increased at 100 mg·kg^-1.The spermatid was damaged seriously at 600 mg·kg^-1,characterized with nuclear membrane and chromatin disappearing.This study suggested that changes of ultrastructure during spermiogenesis can provide theoretical foundation to early pollution prediction of veterinary medicine residue in soil.

• Key words：earthworm  ultrastructure  albendazole  spermatid  ecotoxicology

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