研究报告
沈娥,王平,周豪,孔春雷,马桥,曲媛媛,周集体.一株耐盐苯酚降解菌的分离、鉴定及耐盐机制研究[J].环境科学学报,2013,33(2):377-382
一株耐盐苯酚降解菌的分离、鉴定及耐盐机制研究
- Isolation, identification and the salt-tolerant mechanism of a salt-tolerant strain for phenol degradation
- 基金项目：国家自然科学基金(No.21176040,51078054)
- 作者
- 单位
- 沈娥
- 大连理工大学环境学院, 工业生态与环境工程教育部重点实验室, 大连 116024
- 王平
- 大连百奥泰科技有限公司, 大连 116030
- 周豪
- 大连理工大学环境学院, 工业生态与环境工程教育部重点实验室, 大连 116024
- 孔春雷
- 大连理工大学环境学院, 工业生态与环境工程教育部重点实验室, 大连 116024
- 马桥
- 大连理工大学环境学院, 工业生态与环境工程教育部重点实验室, 大连 116024
- 曲媛媛
- 大连理工大学环境学院, 工业生态与环境工程教育部重点实验室, 大连 116024
- 周集体
- 大连理工大学环境学院, 工业生态与环境工程教育部重点实验室, 大连 116024
- 摘要：从污泥样品中分离得到1株高效的耐盐苯酚降解菌,该菌株能够在温度20~30℃、pH 5.0~9.0和盐度1%~10% 范围内以苯酚为唯一碳源进行生长.通过形态、生理生化特性分析及16S rDNA序列分析,鉴定该菌株为Rhodococcus sp.,命名为W2.在温度30℃、pH=7.0、NaCl 50 g·L-1的条件下,菌株可耐受苯酚的最大浓度为700 mg·L-1.粗酶中检测到的邻苯二酚1,2-双加氧酶活性表明,菌株W2通过邻位开环断裂途径代谢苯酚.底物广谱性考察结果表明,菌株W2能够在高盐条件下利用苯甲酸、水杨酸等多种芳香化合物作为碳源生长.对耐盐机理进行的初步分析表明,细胞内甜菜碱和四氢嘧啶的含量随着盐浓度的增加而增大,说明四氢嘧啶和甜菜碱的积累是菌株W2抵抗高盐度的重要机制.
- Abstract：A salt-tolerant strain for phenol degradation was isolated from active sludge. This strain could utilize phenol as the sole carbon source, and grow in the range of temperature 20 to 30℃, pH 5.0 to 9.0 and 1% to 10% salinity. The strain designated as W2 was identified as Rhodococcus sp. by morphological and biochemical characteristics and 16S rDNA analysis. It could degrade as high as 700 mg·L-1 phenol at temperature 30℃, pH 7.0 and 50 g·L-1 NaCl. The catechol 1,2-dioxygenase activity detected in the extracts indicated that phenol was metabolized through ortho-cleavage pathway in strain W2. Besides phenol, the strain could also degrade various other aromatic compounds, such as benzoate and salicylic acid, under the high salt condition. Determination of the intracellular compatible solutes in strain W2 revealed that the concentrations of betaine and ectoine increased with the increase of salinity. Therefore, the accumulation of betaine and ectoine should be the major mechanism for salt-tolerance in strain W2.
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