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研究报告

武小燕,彭颖,廖伟,李义刚,苏甜,欧瑞康,陈昆慈,李凯彬,聂湘平.布洛芬对黄颡鱼I相代谢酶及其抗氧化系统的影响[J].环境科学学报,2013,33(4):1208-1214

Effects of Ibuprofen on the phase I metabolic enzymes and antioxidant defence system of the Yellow Catfish (Pelteobagrus fulvidraco)

摘要：使用环境相关浓度水平(0.05、0.5、5、50 μg·L^-1)的布洛芬(Ibuprofen,IBU)对黄颡鱼进行水体暴露,研究IBU对黄颡鱼Ⅰ相代谢酶及其抗氧化系统的影响.结果表明,随着暴露时间的延长,IBU对黄颡鱼肝脏Ⅰ相代谢P450酶系的氨基比林-N-脱甲基酶(APND)、红霉素-N-脱甲基酶(ERND)、7-乙氧基-异吩唑酮-脱乙基酶(EROD)和Ⅱ相代谢谷胱甘肽硫转移酶(GST)均表现出先诱导后抑制的作用. 暴露24 h时,IBU浓度为5 μg·L^-1实验组对I相代谢酶APND和ERND的诱导程度最大.当暴露时间达到168 h时,0.5 μg·L^-1浓度组中APND和ERND活性均受到极显著抑制.I相代谢酶EROD活性在暴露24 h后,除0.05 μg·L^-1浓度组无明显变化外,其它浓度组皆受到显著诱导,随着暴露时间延长到168 h时,逐渐恢复到初始水平.GST活性在暴露24 h后,除0.05 μg·L^-1浓度组外,其它浓度组均受到最大程度的诱导,过氧化氢酶(CAT)在暴露168 h时被显著诱导.各浓度组丙二醛(MDA)含量在暴露24 h时较对照组显著下降,72 h时其含量达到最高值.其中,ERND响应较为敏感,适合作为布洛芬暴露的生物标记物.

Abstract：The toxic effects of ibuprofen in environmental levels of 0.05, 0.5, 5 and 50 μg·L^-1 via waterborne exposure on the phase Ⅰmetabolic enzymes and antioxidant defence system of the yellow catfish were investigated. The activities of aminopyrine N-demethylase (APND), erythromycin N-demethylase (ERND), 7-ethoxyresorufin -O-deethylase (EROD) and glutathione-S-transferase (GST) enzymes were induced at the initial stages and gradually inhibited with the extension of exposure time. After 24 h exposure, the highest levels of APND and ERND were detected at the concentration of 5 μg·L^-1. Obvious EROD induction was observed at 24 h in all IBU exposure groups compared to the control except 0.05 μg·L^-1 group and then returned to the normal level after 168 h. The highest value occurred at 24 h for GST and at 168 h for catalase (CAT). The content of malondialdehyde (MDA) decreased significantly after 24 h exposure and reached the highest value at 72 h. ERND was a sensitive parameters and may be used as a potential biomarker for IBU exposure.