研究报告
彭月,朱能武,聂红燕.一株克雷伯氏菌(Klebsiella sp.)Z6的分离及其产电特性研究[J].环境科学学报,2013,33(4):1035-1042
一株克雷伯氏菌(Klebsiella sp.)Z6的分离及其产电特性研究
- Isolation of an electrogen Klebsiella sp.Z6 from anodic biofilm and its electricity-generating characteristics
- 基金项目：国家自然科学基金(No.30800796,31272482);教育部新世纪优秀人才支持计划(No.NCET-11-0166);教育部高校博士点专项基金(No.20070561082);广东省科技计划项目(No.2007A020100001);中央高校基本科研业务费重点项目(No.2009zz0073)
- 作者
- 单位
- 彭月
- 华南理工大学环境与能源学院,广州 510006
- 朱能武
- 1. 华南理工大学环境与能源学院,广州 510006;
2. 工业聚集区污染控制与生态修复教育部重点实验室,广州 510006
- 聂红燕
- 华南理工大学环境与能源学院,广州 510006
- 摘要：产电微生物是驱动微生物燃料电池(Microbial fuel cell,MFC)运行的关键.采用分离培养-MFC产电实验的方法,从以污水处理厂活性污泥为接种物的MFC阳极生物膜中分离到一株产电微生物,命名为Z6.菌株Z6的16S rDNA序列与已知菌株Klebsiella oxytoca An16-2具有100%的同源性,结合该菌生理和形态特征将其初步鉴定为克雷伯氏菌属.菌株Z6接种MFC的产电结果表明,以柠檬酸钠为底物时MFC的最大体积功率密度达到14.35 W·m-3,内阻为400 Ω,显示出较强的电化学活性.通过循环伏安分析和外源AQDS添加实验结果推测,菌株Z6很可能通过分泌电子传递中间体传递电子.
- Abstract：Electrogens is key to boost a microbial fuel cell (MFC). Based on isolation-MFC electricity-generating experiment method, an electrogenic bacterium was isolated from the anodic biofilm of a MFC that was inoculated with aerobic sewage sludge. The electrogenic bacterium was labeled as Z6. The 16S rDNA sequence of Z6 had 100% similarity with known strain Klebsiella oxytoca An16-2. By the consideration of physiological and morphological characteristics, the strain Z6 was preliminarily identified as a member of Klebsiella genus. The electricity-generating results of the MFC inoculated with strain Z6 showed that the maximum power density and internal resistance was 14.35 W·m-3 and 400 Ω, respectively, when citrate was used as the substrate. It was demonstrated that strain Z6 had a relative high electrochemical activity. It can be inferred from the results of cyclic voltammetry analysis and exo-AQDS adding experiment that strain Z6 may transfer electrons through excreting mediators.
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