• 关键词：碳酸盐  磷酸盐  Biohydrogenbacterium R3 sp.nov.  产氢效能  缓冲

• 基金项目：国家高技术研究发展计划(863)项目(No.2006AA05Z109);上海市重点科技攻关项目(No.071605122);上海教委科研项目(No.07zz156);中央高校基本科研业务费专项资金项目(No.DL09AB06)

• 李永峰
• 东北林业大学林学院,哈尔滨 150040

• 陈红
• 东北林业大学林学院,哈尔滨 150040

• 岳莉然
• 东北林业大学林学院,哈尔滨 150040

• 曹逸坤
• 东北林业大学林学院,哈尔滨 150040

• 任南琪
• 哈尔滨工业大学市政环境工程学院,哈尔滨 150090

• 摘要：采用NH₄HCO₃、NaHCO₃、Na₂CO₃、K₂HPO₄、KH₂HPO₄、NaH₂PO₄和Na₂HPO₄作为碳酸盐和磷酸盐缓冲溶液源,研究其对Biohydrogenbacterium R3 sp.nov.产氢发酵效能的影响.研究发现,碳酸盐对Biohydrogenbacterium R3 sp.nov.反应体系中的pH值都具有较好的缓冲作用,而磷酸盐的添加对其产氢效能的促进作用较明显.反应器内添加了Na₂HPO₄与K₂HPO₄的Biohydrogenbacterium R3 sp.nov.的氢气产量与氢气含量达到最高值,分别为1978.56 mL、44.1%与2160.9 mL、45.8%,此时反应器内的pH值分别为3.28和3.41,细胞浓度分别为1.03 g·L^-1和1.21 g·L^-1,添加了Na₂HPO₄的Biohydrogenbacterium R3 sp.nov.的乙醇和乙酸产量分别为4841.49 mg·L^-1和2064.94 mg·L^-1;而添加了Na₂CO₃的氢气产量、氢气含量、细胞浓度与反应器内pH值分别为1064.42 mL、35.96%、1.23 g·L^-1与3.81,此时乙醇、乙酸的产量分别为3862.21 mg·L^-1和1930.86 mg·L^-1.

• Abstract：This study investigated the effects of phosphates and carbonates (NH₄HCO₃, NaHCO₃, Na₂CO₃, K₂HPO₄, KH₂PO₄, NaH₂PO₄ and Na₂HPO₄) on the hydrogen production efficiency and cell growth to Biohydrogenbacterium R3 sp.nov. It was indicated that the carbonates could buffer the pH in reactor better and the phosphates could promote the hydrogen production obviously. When Na₂HPO₄ and K₂HPO₄ were added into the reactor, the hydrogen production and content reached the maximum of 1978.56 mL, 44.1% and 2160.9 mL, 45.8%, respectively. The pH and cell weight achieved 3.28,1.03 g·L^-1 and 3.41, 1.21 g·L^-1, respectively. When Na₂HPO₄ was added into the reactor, the ethanol and acetic acid were 4841.49 mg·L^-1 and 2064.94 mg·L^-1, respectively, while when Na₂CO₃ was added into the reactor, the hydrogen production, hydrogen content, cell weight, pH, ethanol and acetic acid were 1064.42 mL, 35.96%, 1.23 g·L^-1, 3.81, 3862.21 mg·L^-1 and 1930.86 mg·L^-1, respectively.

• Key words：phosphates  carbonates  Biohydrogenbacterium R3 sp.nov.  biohydrogen production  buffer

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