• 关键词：镰刀菌(Fusarium sp. HJ01)  对氯苯酚  生物降解  重金属

• 基金项目：浙江省科技计划项目(No.2012C23064)

• 潘俊雅
• 浙江工商大学环境科学与工程学院, 杭州 310035

• 李济吾
• 浙江工商大学环境科学与工程学院, 杭州 310035

• 摘要：为了解Cd²⁺对镰刀菌(Fusarium sp. HJ01)降解对氯苯酚(4-CP)的影响,研究了Cd²⁺共存时,浓度、pH、碳源与氮源对镰刀菌降解4-CP特性的影响,并分析了其降解动力学与机理.结果表明,在降解温度、4-CP浓度等相同时,Cd²⁺共存对镰刀菌降解4-CP有一定的抑制作用.Cd²⁺浓度小于3.0 mg·L^-1时,随着4-CP浓度的增大,镰刀菌对4-CP的降解速率变慢.镰刀菌降解4-CP的适宜pH为4.外加碳源蔗糖(3.0 g·L^-1)与外加NH₄Cl(3.0 g·L^-1)均使镰刀菌降解4-CP速度有所减缓.在Cd²⁺浓度0.1~3.0 mg·L^-1时,镰刀菌降解4-CP符合零级动力学方程.Cd²⁺共存时只对4-CP的降解速率产生一定的抑制作用,而对其降解途径可能无显著影响.

• Abstract：The effects of concentration, pH, carbon and nitrogen source on degradation of p-chlorophenol (4-CP) by Fusarium sp. HJ01 with co-existence of Cd²⁺ were investigated. The kinetic and mechanism of 4-CP degradation by Fusarium sp. HJ01 were discussed. It was concluded that cadmium (Cd²⁺) had a restraining effect on degradation of p-chlorophenol by Fusarium sp. HJ01 in the same temperature and concentration of 4-CP. The rate of 4-CP biodegradation with Cd²⁺ coexistence was decreasing with the increasing of 4-CP concentration while Cd²⁺ of concentration was less 3.0 mg·L^-1. The suitable pH for degradation of 4-CP by Fusarium sp. HJ01 was equal to 4. Addition of 3.0 g·L^-1 carbon (C₁₂H₂₂O₁₁) and nitrogen source could weaken the rate of 4-CP degradation by Fusarium sp. HJ01. The kinetic of 4-CP biodegradation could well accord with the zero order equation for 4-CP as the sole carbon source with 0.1~3.0 mg·L^-1 Cd²⁺ coexistence. Cd²⁺ could decrease the rate of 4-CP degradation, but might not change the pathway of 4-CP degradation.

• Key words：Fusarium sp. HJ01  p-chlorophenol  biological degradation  heavy metals

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