• 关键词：零价铁  荧光假单胞菌  直接耐晒黑19  偶氮染料  废水处理

• 基金项目：国家重大科技专项(No.2009ZX07211-005);湖南省教育厅开放基金(No.10K064);湘潭大学博士启动基金(No.08QDZ31)

• 肖利平
• 湘潭大学环境工程系, 湘潭 411105

• 郭燕
• 广东环境保护工程职业学院, 佛山 528216

• 邓志毅
• 湘潭大学环境工程系, 湘潭 411105

• 江栋
• 环境保护部华南环境科学研究所, 广州 510655

• 刘永
• 环境保护部华南环境科学研究所, 广州 510655

• 摘要：采用荧光假单胞菌(Pseudomonas fluorescen,简称pf菌)与Fe⁰微粒构建Fe⁰/pf菌联合体系,处理了含偶氮类染料-直接耐晒黑(C.I. Direct black19,简称DB19)废水.在中温((35±2) ℃)条件下,比较了纯pf菌、纯Fe⁰微粒和Fe⁰/pf菌联合体系的脱色能力,并探讨了联合体系中供氧条件、初始pH值、Fe⁰投加量和染料初始浓度等因素对DB19脱色的影响.结果表明,在微需氧、菌接种量为5%(体积比)的条件下,染料初始浓度为100 mg·L^-1、初始pH=7.0、Fe⁰投加量为500 mg·L^-1时,联合体系内脱氢酶活性最高,处理约30 h后可实现90%以上的脱色率;与纯pf菌比较,Fe⁰/pf菌联合体系达到该脱色率的时间可提前约40 h.UV-vis和FT-IR光谱分析表明,联合体系中DB19染料的脱色过程是通过偶氮键断裂来实现的,而且部分含苯环或萘环结构的中间产物也可被降解.

• Abstract：The dye C.I. Direct black19 in the wastewater, denoted by DB19, was degraded by the strain Pseudomonas fluorescence with Fe⁰ in the system containing Fe⁰ micro-particles. The DB19 decolorization of P. fluorescence, Fe⁰ micro-particles, and P. fluorescence with Fe⁰ were compared at (35±2) ℃. The effects of oxygen supply mode, initial dye concentration, initial pH and dosage of Fe⁰ on decolorization rate were discussed. The results showed that the activity of dehydrogenase was the highest, and the decolorization rate of DB19 was up to 90% under the condition of initial DB19 concentration 100 mg·L^-1, initial pH 7.0, dosage of Fe⁰ 500 mg·L^-1 and inoculation volume of the strain 5% (V/V) for 30 h in microaerophilic environment. The time for treatment by P. fluorescence with Fe⁰ was about 40 hours shorter than that solely by P. fluorescence. According to UV-vis and FT-IR analysis, the decolorization of DB19 was mainly due to the rupture of azo bond, and mid-productions including benzene or naphthalene could also be partially decomposed by P. fluorescence with Fe⁰.

• Key words：zero-valent iron  Pseudomonas fluorescence  C.I. Direct black19  azo dye  wastewater treatment

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