• 关键词：异养硝化  好氧反硝化  碳源  氮源  不动杆菌

• 基金项目：国家自然科学基金(No.51078252);山西省国际合作计划项目(No.2010081018);山西省自然科学基金(No.2010011016-1);山西省教育厅科技发展计划项目(No.20091007);山西省教育厅研究生优秀创新项目(No.20093045)

• 宋宇杰
• 南京农业大学有机农业与有机食品研究所, 南京 210095

• 李屹
• 国家水专项管理办公室, 北京 100029

• 刘玉香
• 太原理工大学环境科学与工程学院, 太原 030024

• 和文龙
• 南京农业大学有机农业与有机食品研究所, 南京 210095

• 摘要：从焦化废水活性污泥中筛选到一株高效脱氮细菌,命名为Acinetobacter sp. Y1.本实验对菌株Y1在不同碳源、氮源、碳氮比及底物浓度下的脱氮特性进行了研究,结果表明,菌株Y1可以利用氨氮、亚硝氮和硝氮生长,不能利用羟胺;以氨氮为唯一氮源进行硝化作用时,柠檬酸钠和乙酸钠是最佳碳源,最佳碳氮比为15,菌株Y1可降解高浓度氨氮,在36h内将400 mg·L^-1氨氮全部去除,1600 mg·L^-1氨氮的去除率可达21.3%,最大降解速率随着初始氨氮浓度的升高而增大.以硝氮或亚硝氮为唯一氮源进行反硝化时,菌株Y1可以适应高浓度氮源但不能完全去除氮源,当碳氮比为20,经36h培养硝氮和亚硝氮的去除率均达到100%.

• Abstract：Acinetobacter sp. Y1, a high effective heterotrophic nitrification bacterium, was isolated from the activated sludge of a coking wastewater treatment facility. The characteristics of nitrogen removal by strain Y1 at different carbon sources, C/N ratios, nitrogen sources and substrate concentrations were investigated. The result shows that Acinetobacter sp. Y1 can remove different forms of nitrogen sources. The most efficient ammonium removal and growth rate for Y1 was occurred at C/N=15 when sodium citrate was supplemented as the carbon source, ammonium removal rate reached 99% and OD_600max was 1.432. High concentration of ammonium ranging from 400 mg·L^-1 to 1600 mg·L^-1 could be degraded well by strain Y1. The most efficient nitrogen removal was occurred at C/N=20 when nitrite or nitrate was treated as a sole nitrogen source.

• Key words：heterotrophic nitrification  aerobic denitrification  carbon source  nitrogen source  Acinetobacter

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