• 关键词：活性污泥  偶氮染料  胞外聚合物(EPS)  三维荧光光谱

• 基金项目：国家自然科学基金(No.51008112);国家重大科技专项(No.2012ZX07101-003)

• 操家顺
• 1. 河海大学浅水湖泊综合治理与资源开发教育部重点实验室, 南京 210098;
  2. 河海大学环境学院, 南京 210098

• 朱哲莹
• 1. 河海大学浅水湖泊综合治理与资源开发教育部重点实验室, 南京 210098;
  2. 河海大学环境学院, 南京 210098

• 方芳
• 1. 河海大学浅水湖泊综合治理与资源开发教育部重点实验室, 南京 210098;
  2. 河海大学环境学院, 南京 210098

• 李宇昇
• 1. 河海大学浅水湖泊综合治理与资源开发教育部重点实验室, 南京 210098;
  2. 河海大学环境学院, 南京 210098

• 胡海兰
• 1. 河海大学浅水湖泊综合治理与资源开发教育部重点实验室, 南京 210098;
  2. 河海大学环境学院, 南京 210098

• 摘要：采用序批式反应器(SBR)考察了偶氮染料浓度对活性污泥的污染物去除性能及胞外聚合物(Extracellular polymeric substances,EPS)的影响.结果表明,COD、染料及营养物的去除率均随着进水染料浓度的增加而下降,但进水染料浓度对EPS的影响却呈现不同的趋势.当进水染料浓度为5~40 mg·L^-1时,EPS含量随着染料浓度的增加而增加;当进水染料浓度超过40 mg·L^-1时,EPS含量却随着染料浓度的增加而减少.染料的加入导致活性污泥EPS中蛋白质的含量增加,且其变化趋势与EPS变化一致;EPS中腐殖酸的浓度低于蛋白质浓度;而EPS中多糖的浓度最低,为8 mg·g^-1.三维荧光光谱结果显示,不同染料浓度下EPS荧光吸收峰数量及位置相同,分别为类蛋白峰(λ_Ex/λ_Em=240 nm/375~394 nm)和类富里酸类峰(λ_Ex/λ_Em=270 nm /410~416 nm),但两个吸收峰的荧光强度不同.

• Abstract：In this study, a sequencing batch reactor (SBR) was used to investigate the nutrient removal and extracellular polymeric substances (EPS) production by activated sludge with different azo dye concentrations. The results indicated that the chemical oxygen demand (COD), azo dye and nutrient removal efficiencies all decreased with the increase in dye concentration. However, the influence of dye concentration on EPS production showed a different trend. When the dye concentration was 5~40 mg·L^-1, EPS concentration increased with increasing dye concentration. However, the EPS concentration decreased when the concentration of influent dye exceeded 40 mg·L^-1. Furthermore, azo dye led to more protein production than humus, and the protein concentration showed the same trend with that of total EPS. Polysaccharides was the least fraction in EPS, which was 8 mg·g^-1. The spectral information about the chemical compositions of EPS was presented by the three-dimensional excitation-emission matrix (EEM). They were similar in the peak locations, but had different fluorescence intensities. Two major components of EPS were identified as fulvic acid-like at λ_Ex/λ_Em= 270 nm /410~416 nm and proteins at λ_Ex/λ_Em=240 nm /370~394 nm.

• Key words：activated sludge  azo dye  extracellular polymeric substances (EPS)  three-dimensional excitation-emission matrix (EEM)

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