• 关键词：嗜酸性氧化亚铁硫杆菌  接种密度  铁沉淀率  次生铁矿物  矿相

• 基金项目：国家自然科学基金 (No.40930738,21277071);山西农业大学科技创新基金项目(No. 201301)

• 刘奋武
• 1. 山西农业大学资源与环境学院环境工程实验室, 太谷 030801;
  2. 南京农业大学资源与环境科学学院环境工程系, 南京 210095

• 王敏
• 湖北大学资源环境学院环境工程系, 武汉430062

• 卜玉山
• 山西农业大学资源与环境学院环境工程实验室, 太谷 030801

• 崔春红
• 南京农业大学资源与环境科学学院环境工程系, 南京 210095

• 梁剑茹
• 南京农业大学资源与环境科学学院环境工程系, 南京 210095

• 周立祥
• 南京农业大学资源与环境科学学院环境工程系, 南京 210095

• 摘要：嗜酸性氧化亚铁硫杆菌(A.ferrooxidans)促进次生铁矿物形成的现象在酸性煤矿废水(ACMD)的治理领域具有重要意义.本研究探索了A. ferrooxidans接种密度在酸性硫酸盐环境(9K培养基)中对Fe²⁺氧化率、总Fe沉淀率及矿物产生量的影响,同时考察了矿物合成体系矿相的变化情况.结果表明,当体系A. ferrooxidans接种密度为0.27×10⁶~5.40×10⁷ cells·mL^-1时,溶液中Fe²⁺需60~12 h氧化完全.培养至60 h,上述体系总Fe沉淀率分别达到10.7%~35.9%.不同接种体系Fe²⁺同时氧化完全时,沉淀单位质量Fe而转化的次生铁矿物量随着接种密度的增加而增大.例如,A. ferrooxidans接种密度分别为1.35×10⁶、2.70×10⁶、8.10×10⁶和1.62×10⁷ cells·mL^-1的处理在Fe²⁺同时完全氧化时刻,Fe沉淀率分别为17.6%、20.0%、24.1% 和26.5%,且沉淀1 g Fe转化的次生铁矿物量分别为2.04、2.10、2.17与2.27 g.结晶度较差的施氏矿物是次生铁矿物合成初期产生的唯一矿相,Fe²⁺完全氧化时,矿物相为施氏矿物与结晶度好的黄铁矾矿物的混合物.

• Abstract：The formation of secondary iron minerals facilitated by A. ferrooxidans is of important engineering significance in the field of acidic coal mine drainage (ACMD) treatment. In this study, secondary iron minerals were synthesized by A. ferrooxidans in the pH 2.5 sulfate-rich environment (modified 9K liquid medium).The effect of A. ferrooxidans inoculation density on Fe²⁺ oxidation rate, total Fe precipitation rate, and mineral weight in secondary iron minerals biosynthesis system were investigated. In addition, mineral phase transformation during biosynthesis process was also studied. The results showed that ferrous ions could be completely oxidized by A. ferrooxidans at 60~12 h when A. ferrooxidans inoculation density were 0.27×10⁶~5.40×10⁷ cells·mL^-1 in systems. The total Fe precipitation rates were 10.7%~35.9% in above systems after 60 h incubation. The ratio of the amount of resulting secondary iron minerals to precipitated Fe in the systems increased with the increase of the inoculation density of A. ferrooxidans when Fe²⁺ was completely oxidized. For example, when A. ferrooxidans inoculation density was 1.35×10⁶, 2.70×10⁶, 8.10×10⁶, and 1.62×10⁷ cells·mL^-1, total Fe precipitation rates were 17.6%, 20.0%, 24.1% and 26.5%, in correspondence to 2.04, 2.10, 2.17, and 2.27 g secondary iron minerals formed from 1 g precipitated Fe at ferrous iron entire oxidation moment in the systems, respectively. A poorly crystalline schwertmannite was the only mineral phase found in system during initial stage of secondary iron minerals biosynthesis. However, mineral phase was the mixture of schwertmannite and jarosite when ferrous ions were completely oxidized in the systems.

• Key words：A. ferrooxidans  inoculation density  Fe precipitation rate  secondary iron minerals  mineral phase

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