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研究论文

刘莉华,刘淑杰,陈福明,杨小龙,杨春平,吴秉奇,张淼,赵晶晶.接种内生细菌对龙葵吸收积累镉的影响[J].环境科学学报,2013,33(12):3368-3375

Effect of endophytic bacteria inoculation on cadmium uptake in Solanum nigrum L.

杨春平
1. 南昌航空大学环境与化学工程学院, 南昌 330063;
3. 浙江省固体废物处理与资源化重点实验室, 浙江工商大学环境科学与工程学院, 杭州 310018;
4. 湖南大学环境科学与工程学院, 长沙 410082

摘要：通过在LB培养基中加入不同浓度的镉，从镉超富集植物龙葵植株内分离到3株很强的镉抗性内生菌株N1、N2、N4，最高耐受镉浓度达300 mg·L^-1.通过生理生化鉴定和16S rDNA序列分析，初步确定N1为芽孢杆菌属（Bacillus sp.）细菌，N2为肠杆菌属（Enterobacter sp.）细菌，N4为巨大芽孢杆菌（Bacillus megaterium）.将龙葵种植在含有一定镉（50 mg·kg^-1）的土壤中进行盆栽试验，以灌根的方式分别接种分离到的3株细菌及其混菌组合，并对其株高、生物量及镉吸收能力进行分析.结果显示，N4菌株处理的龙葵叶、茎、根部镉含量比不接菌对照分别增加32.94%、14.50%、23.44%，差异显著（p<0.05）；接种N1+N2+N4混菌的处理龙葵叶、茎和根干重分别比不接菌对照高出118.22%、109.83%和113.01%，植株地上和地下镉吸收总量分别增加109.53%和83.01%，差异显著.试验表明供试菌株能显著促进植株生长，强化龙葵吸收土壤中镉的能力.

Abstract：Three strong cadmium-resistance endophytic bacterium N1, N2 and N4 were isolated from cadmium hyperaccumulator Solanum Nigrum L. through adding various concentrations of Cd in the media. The bacteria were preliminarily identified as Bacillus sp. (N1), Enterobacter sp. (N2) and Bacillus megaterium (N4), respectively by morphological and physiological characteristics and 16S rRNA sequential analysis. Pot culture test of Solanum Nigrum L. was carried out in soil with a cadmium concentration of 50 mg·kg^-1, and the above strains of different groups were inoculated in the Solanum Nigrum L. land and the height, biomass and absorption capacity for cadmium of Solanum Nigrum L. were analyzed. The results showed that cadmium contents in leaf, stem and root in the Solanum Nigrum L. land treated with strain N4 increased significantly by 32.94%, 14.50% and 23.44%, respectively (p<0.05), compared with the control. In the Solanum Nigrum L. land treated with strains N1+N2+N4, the dry weight of leaf, stem and root increased by 118.22%, 109.83% and 113.01%, respectively, and the cadmium content in the rhizospheric and amount of cadmium uptake increased by 109.53% and 83.01%, respectively. The test indicated the endophytic bacterium could promote plant growth significantly, and strengthen the cadmium absorption from soil by Solanum nigrum L.