• 关键词：聚磷微生物  产碱杆菌  筛选鉴定  特性  固定化

• 基金项目：福建省财政厅资助项目（No.20130421）；福建省大学生创新创业训练项目（No.sjcxcy2012-022）；福建师范大学拔尖人才训练项目（生物学拔尖201004）

• 庄志刚
• 福建师范大学生命科学学院, 福州 350108

• 韩永和
• 1. 福建师范大学生命科学学院, 福州 350108;2. 南京大学环境学院生物地球化学与环境修复实验室, 南京 210046

• 章文贤
• 福建师范大学生命科学学院, 福州 350108

• 周志华
• 福建师范大学生命科学学院, 福州 350108

• 陈佳兴
• 福建师范大学生命科学学院, 福州 350108

• 李敏
• 福建师范大学生命科学学院, 福州 350108

• 摘要：利用经典的微生物筛选方法，从福州市闽侯县上街镇高岐村某排污口淤泥中分离出1株高效聚磷菌，并结合16S rRNA基因序列分析进行了菌株鉴定. 结果表明，该菌株为产碱杆菌，将其命名为Alcaligenes sp. ZGED-12.理化因素实验显示，在以乙酸钠为碳源、NH₄Cl为氮源，当C/N为3：1，pH为8.0，温度和摇床转速分别为35 ℃和100 r · min^-1时，该菌株的生长状态最好，对磷的去除能力也最强，最高除磷率可达80%. 此外，该菌株能够耐受较高浓度的磷，当磷浓度超过45 mg · L^-1时会产生抑制效应. 同时，以聚乙烯醇（PVA）和海藻酸盐（SA）制备了聚磷微生物固定化小球，并考察了菌球对氮磷废水的净化效果. 结果表明，氮磷的去除包括固定化材料的吸附作用及微生物的生长利用和/或贮存，显示出了良好的应用前景.

• Abstract：A phosphorus-accumulating bacteria was isolated from a drain outlet located in Gaoqi Village, Shangjie Town, Minhou County, Fuzhou, China. The strain, named Alcaligenes sp. ZGED-12, was obtained by classic methods for screening microorganisms together with 16S rRNA gene analysis. The culture conditions showed that the best carbon source and nitrogen source were sodium acetate and NH₄Cl, respectively, the optimal pH was 8.0, the optimal temperature was 35 ℃, the best C/N was 3:1, and the optimal shaking rate was 100 r · min^-1. Under these optimized conditions, the maximum removal efficiency for phosphorus was higher than 80%. The strain has a high tolerance for phosphorus, but its growth was inhibited when the concentration of phosphorus was higher than 45 mg · L^-1. Meanwhile, the immobilized phosphorus-accumulating organisms beads (IMOBs) were prepared, and the purification efficiency of IMOBs for nitrogen/ phosphorus contaminated wastewater was investigated. The results showed that removal for nitrogen and phosphorus was due to absorption on the immobilized materials and uptake/metabolism of microorganisms, indicating a good prospective of IMOBs in the treatment of wastewater.

• Key words：phosphorus-accumulating organisms(PAOs)  Alcaligenes sp.  screening and identification  characteristics  immobilization

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