• 关键词：厌氧污泥  生化产CH₄势  T-RFLP

• 基金项目：西门子（中国）有限公司资助项目；公益性行业（农业）科研项目（No.201303101）；国家高技术研究发展计划（No.2012AA063502）

• 董慧峪
• 中国科学院生态环境研究中心环境水质学国家重点实验室, 北京 100085

• 季民
• 天津大学环境科学与工程学院, 天津 300072

• 摘要：选用NaAc为基质，对AP、DH两污水处理厂的厌氧污泥进行了生化产甲烷（CH₄）势实验（BMP）.经改进的Gompertz模型和Michaelis-Menten模型回归分析可知，尽管AP、DH两厂厌氧污泥的最大比产气速率数值接近（67.93、62.65 mL·g^-1·d^-1，以VSS计），但对基质NaAc的半饱和常数K_m差异较大（1517和801 mg·L^-1），DH厌氧污泥对基质的亲和力较好.BMP实验结束时DH厌氧污泥中的ORP位于-310~-373 mV之间，投加基质时CH₄回收率高于80%，均优于AP厌氧污泥.T-RFLP与SEM镜检结果与BMP实验结果较为吻合，AP厌氧污泥中产CH₄功能菌群群落丰度较低，Diverse菌群相对丰度达45%，而DH厌氧污泥中产CH₄功能菌种群密集，Diverse菌群相对丰度仅为7%.DH厌氧污泥中产CH₄功能菌以产CH₄髦毛菌Methanosaeta spp.（280 bps）为主，同时存在产CH₄微菌Methanomicrobiaceae（80 bps）、RC-I（389 bps）和产CH₄杆菌Methanobacteriacea（88 bps）.

• Abstract：AP and DH anaerobic sludge, sampled from AP and DH wastewater treatment plants respectively, were tested with biological methane potential (BMP) method adopting NaAc as substrate. After the regression analysis with modified Gompertz and Michaelis-Menten model, it was found that although the maximum specific CH₄ production rates of AP and DH anaerobic sludge were similar (67.93 and 62.65 mL·g^-1·d^-1), the half-saturation constants K_m differed obviously (1517 and 801 mg·L^-1), indicating that DH anaerobic sludge exhibited a higher affinity for substrate. The ORP values in DH-BMP ranged from -310 mV to -373 mV, which were lower than those in AP-BMP. With the addition of NaAc, the recoveries of CH₄ in DH-BMP were > 80%, higher than those in AP-BMP. The T-RFLP and SEM analysis results were in accordance with BMP test. The microbial community abundance of Methanogenus in AP anaerobic sludge was lower with diverse bacteria exceeding 45% while the diverse bacteria in DH anaerobic sludge just reached 7%. Apart from Methanosaeta spp. (280 bps) dominating the Methanogenus, Methanomicrobiaceae (80 bps), RC-I (389 bps) and Methanobacteriacea (88 bps) were also observed in DH anaerobic sludge.

• Key words：anaerobic sludge  biological methane potential  T-RFLP

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