• 关键词：SO₂  线粒体  细胞色素C氧化酶  ATP合酶  PGC-1α  NRF1  mtTFA

• 基金项目：国家自然科学基金（No.21007036）；山西省自然科学基金（No.2012011036-6）；山西省高校优秀青年学术带头人支持计划（No.20120303）

• 秦国华
• 山西大学环境与资源学院, 太原 030006

• 霍雅俊
• 山西大学环境与资源学院, 太原 030006

• 桑楠
• 山西大学环境与资源学院, 太原 030006

• 摘要：采用Wistar大鼠作为模型进行整体动物动式吸入染毒，在大鼠吸入不同浓度SO₂（0、7、14、28 mg·m^-3，7 d，6 h·d^-1）后，采用荧光定量PCR技术检测心肌细胞中由线粒体DNA（mtDNA）编码的细胞色素C氧化酶和ATP合酶的几种亚基及调控线粒体呼吸链组分的核转录因子PGC-1α、NRF-1和mtTFA的mRNA水平.结果发现，SO₂染毒后，随着SO₂浓度的升高，大鼠心肌中细胞色素C氧化酶3种亚基（CO1、CO2、CO3）和ATP合酶两种亚基（ATP6&8） mRNA表达水平均显著降低，调控基因PGC-1α、NRF1和mtTFA的mRNA表达水平也显著降低.提示大鼠吸入SO₂后，可能通过降低心肌中PGC-1α表达，影响其与 NRF1 结合及对mtTFA调控，进一步抑制线粒体基因组的转录，最终可能使大鼠心肌线粒体的氧化磷酸化功能受损，进而引起心衰等心血管疾病.

• Abstract：Male Wistar rats were housed in exposure chambers and treated with 0, 7, 14, and 28 mg·m^-3 SO₂ for 6 h·d^-1 for 7 days. Analyses of mitochondrial oxidative phosphorylation complex and three mitochondrial transcript factors transcription, including NRF1, mtTFA and PGC-1α, were performed by real time PCR. Our results showed that mRNA expression of CO1, CO2, CO3, and ATP6 & 8 significantly decreased in rats after SO₂ exposure. Moreover, it was accompanied by depression of three transcript factors. It was indicated that SO₂ inhalation induced rat myocardial cell mitochondrial dysfunction might be related to the PGC-1α pathway, and the diminished transcriptional levels of electron transport chain subunits. It might cause the damage of rat myocardial cell mitochondrial oxidative phosphorylation function and even some cardiovascular disease, such as heart failure.

• Key words：SO₂  mitochondria  cytochrome c oxidase  ATPase  PGC-1α  NRF1  mtTFA

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