• 关键词：转速  镁离子  氧化亚铁硫杆菌  次生铁矿物  矿相

• 基金项目：国家自然科学基金项目（No.41371476，21277071）；山西农业大学博士科研启动基金项目（No.2012YJ06）；山西农业大学科技创新基金项目（No.201301）

• 刘奋武
• 山西农业大学资源环境学院环境工程实验室, 太谷 030801

• 高诗颖
• 山西农业大学资源环境学院环境工程实验室, 太谷 030801

• 卜玉山
• 山西农业大学资源环境学院环境工程实验室, 太谷 030801

• 崔春红
• 南京农业大学资源与环境科学学院环境工程系, 南京 210095

• 周立祥
• 南京农业大学资源与环境科学学院环境工程系, 南京 210095

• 摘要：探析培养转速与镁离子浓度对氧化亚铁硫杆菌生物合成次生铁矿物的影响对酸性矿山废水（AMD）治理具有一定的工程指导意义.本研究通过摇瓶实验，研究了Mg²⁺浓度分别为48与4.8 mg·L^-1，其它元素组成与富含Fe与SO₄^2-的9K液体培养基一致的体系在180 r·min^-1 与100 r·min^-1转速条件下氧化亚铁硫杆菌催化合成次生铁矿物过程.考察了不同次生铁矿物合成体系pH、Fe²⁺氧化率、总Fe沉淀率及次生铁矿物矿相等相关指标.研究结果表明，在180 r·min^-1 的培养条件下，Mg²⁺浓度分别为4.8与48 mg·L^-1两体系培养48 h后，pH 从原始的2.50 分别降低至2.07与2.12，Fe²⁺均可在48 h内实现完全氧化.Fe²⁺完全氧化时，Mg²⁺浓度为4.8 mg·L^-1体系总Fe沉淀率为37.4%，合成的次生铁矿物均匀分散于溶液中，而Mg²⁺浓度为48 mg·L^-1体系中，总铁沉淀率仅为31.7%，且70%的矿物牢固粘附于摇瓶底部.培养转速为100 r·min^-1时，Mg²⁺浓度分别为4.8与48 mg·L^-1两体系经过72 h培养后，pH 均从原始的2.50降低至2.21与2.17.Fe²⁺需要72 h才能被完全氧化，两体系总Fe沉淀率分别仅为21.3%与23.0%，产生的次生铁矿物几乎全部牢固粘附于摇瓶底部.本研究所有体系产生的次生铁矿物均为黄铁矾与施氏矿物的混合物.研究结果可为生物合成次生铁矿物工艺的优化及其在酸性矿山废水治理领域的有效应用提供必要的参数支撑.

• Abstract：Understanding the effect of shaker speed and magnesium concentration on the formation of biogenic secondary iron minerals facilitated by A. ferrooxidans is of important engineering significance in the field of acid mine drainage (AMD) treatment. In this study, secondary iron minerals was synthesized in acidic Fe and SO₄^2--rich solution through the oxidation of ferrous iron by A. ferrooxidans and subsequent hydrolysis in 9K liquid medium containing 48 or 4.8 mg·L^-1 magnesium ions at shaker speed of 180 r·min^-1 or 100 r·min^-1. The pH, Fe²⁺ oxidation rate, total Fe precipitation rate, secondary iron mineral phase, and mineral morphology in different treatments were investigated. Results showed that solution pH decreased from an initial 2.50 to final 2.07 or 2.12, and Fe²⁺ achieved complete oxidation after 48 h incubation at 180 r·min^-1 for 4.8 or 48 mg·L^-1 magnesium ions systems, respectively. When Fe²⁺ was completely oxidized, total Fe precipitation efficiency was 37.4% in 4.8 mg·L^-1 magnesium ions systems and secondary iron minerals uniformly dispersed in the solution. But in 48 mg·L^-1 magnesium ions systems, total Fe precipitation efficiency was 31.7%, and 70% of secondary iron minerals were closely adhered to the flask bottom. Solution pH decreased from an initial 2.50 to final 2.21 or 2.17, and the Fe²⁺ achieved complete oxidation after 72 h incubation at 100 r·min^-1 for 4.8 or 48 mg·L^-1 magnesium ion systems, respectively. Total Fe precipitation efficiency was 21.3% for 4.8 mg·L^-1 magnesium ion system and 23.0% for 48 mg·L^-1 magnesium ion system. Likewise, Fe²⁺ achieved complete oxidation, but secondary iron mineral was closely adhered to the flask bottom in both systems. In this study, mineral phase were identified as the mixture of jarosite and schwertmannite in all treatments. The data obtained from this study were helpful in the engineering application of secondary iron minerals biosynthesis and its use in AMD treatment.

• Key words：culture speed  magnesium ions  A. ferrooxidans  secondary iron minerals  mineral phase

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