• 关键词：丝状菌  污泥沉降性  EPS  污泥密度  污泥含磷量  温度  A/O除磷  PCR-DGGE

• 基金项目：国家自然科学基金项目（No.50878108）；陕西省自然科学基金项目（No.2007E201）

• 聂琨
• 西安建筑科技大学 西北水资源与环境生态教育部重点实验室, 西安 710055

• 陈希
• 西安建筑科技大学 西北水资源与环境生态教育部重点实验室, 西安 710055

• 袁林江
• 西安建筑科技大学 西北水资源与环境生态教育部重点实验室, 西安 710055

• 南亚萍
• 西安建筑科技大学 西北水资源与环境生态教育部重点实验室, 西安 710055

• 郭明
• 西安建筑科技大学 西北水资源与环境生态教育部重点实验室, 西安 710055

• 摘要：本试验平行运行了两套A/O生物强化除磷系统，通过观测温度诱发的污泥性状及微生物群落结构的变化，探索了温度对污泥沉降性能的影响及机理.试验结果表明，温度从20℃升至25℃能够有效改善污泥的沉降性能，而从20℃降至15℃可以使污泥沉降性急剧恶化.升温和降温均可导致EPS中蛋白质含量在短期内急剧增加.高温（25℃）能够抑制丝状菌的生长，而低温（15℃）会刺激Thiothrix II型丝状菌大量增殖.SPSS相关性分析显示，丝状菌的数量变化不大时，污泥含磷量和不可挥发性固体含量对沉降性影响显著，EPS的增加不利于污泥沉降，污泥密度与污泥沉降性无相关性.聚合酶链式反应-变性梯度凝胶电泳（PCR-DGGE）分析表明，不同温度会导致不同优势菌的生长.受高温影响较大的细菌为拟杆菌门（Bacteroidetes）和变形菌门（Proteobacteria），受低温影响较大的细菌为变形菌门（Proteobacteria）、拟杆菌门（Bacteroidetes）和放线菌门（Actinobacteria）.

• Abstract：The effect and mechanism of temperature on sludge settleability was investigated by observing temperature-induced changes in sludge properties and microbial community structure in two sets of parallel-operated A/O-enhanced biological phosphate removal systems. The results indicated that the sludge settleability improved as temperature rose from 20℃ to 25℃, but deteriorated dramatically as temperature dropped from 20℃ to 15℃. In both warming and cooling processes, protein content in extracellular polymeric substances increased sharply in the short term. High temperature (25℃) inhibited the growth of filamentous bacteria, whereas low temperature (15℃) stimulated the sharp proliferation of Thiothrix II filamentous bacteria. Univariate linear correlation by SPSS showed that sludge settleability was remarkably affected by the phosphorus and non-volatile solid content of the sludge when the population of filamentous bacteria changed slightly. The increase of EPS inhibited sludge settling, and no correlation was found between sludge density and sludge settleability. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis suggests that different temperatures can lead to different dominant bacteria and influence sludge settleability. The types of bacteria considerably influenced by high temperature were Bacteroidetes and Proteobacteria, and those significantly influenced by low temperature were Proteobacteria, Bacteroidetes and Actinobacteria.

• Key words：filamentous bacteria  sludge settleability  EPS  sludge density  phosphorus content of sludge  temperature  A/O phosphorus removal  PCR-DGGE

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