• 关键词：C/N  微生物C  微生物N  紫色土

• 基金项目：国家自然科学基金（No.31200404）；中国科学院成都山地所山地表生过程与生态调控重点实验室开放基金（No.Y2R20050DD）；中科院地理所"秉维优秀青年人才"项目（No.2012RC202）

• 高扬
• 1. 中国科学院地理科学与资源研究所, 中国科学院生态系统网络观测与模拟重点实验室, 北京 100101;2. 中国科学院成都山地灾害与环境保护研究所, 成都 610041

• 徐亚娟
• 中南林业科技大学, 长沙 410000

• 陈维梁
• 福建农林大学生命科学学院, 福州 350002

• 朱波
• 中国科学院成都山地灾害与环境保护研究所, 成都 610041

• 汪涛
• 中国科学院成都山地灾害与环境保护研究所, 成都 610041

• 于贵瑞
• 中国科学院地理科学与资源研究所, 中国科学院生态系统网络观测与模拟重点实验室, 北京 100101

• 摘要：以紫色土坡耕地为研究对象，综合探讨不同坡度变化下植被/裸地小区以及长期不同施肥处理下紫色土坡耕地土壤 C、N与微生物C、N变化及其耦合特征.结果表明：植被覆盖小区总有机碳（TOC）变化为49.0~63.9 g·kg^-1， 裸地TOC变化为47.4~50.8 g·kg^-1，并随坡度增加逐渐下降；植被小区微生物碳（MBC）为0.9~3.45 g·kg^-1，而裸地小区MBC仅为0.1~0.68 g·kg^-1.不同施肥处理下坡耕地土壤TOC为57.0~64.5 g·kg^-1，施加有机肥处理土壤的TOC含量高于其他施肥方式.植被小区总氮（TN）为2.6~4.2 g·kg^-1，裸地小区TN含量为1.6~5.5 g·kg^-1，植被小区微生物氮（MBN）高于裸地小区；不同施肥处理下TN含量随土层深度增加呈显著下降，有机肥和秸秆与化肥配施相比单一施加氮肥对土壤MBN的增加更为显著.有机肥及其与化肥配施下土壤C/N比为10~17，而秸秆及其与化肥配施土壤C/N比为8~13，单施氮肥为12左右，增施有机肥和秸秆还田有利于保持土壤 C/N 的稳定提高；同时，施肥有利于提高微生物熵，增加土壤中活性有机碳的比例；紫色土坡耕地MBC和TN具有显著的耦合特征（R²=0.66）.

• Abstract：The aim of this paper is to discuss the changes and coupling of C and N in the hillslope cropland of purple soil and microbial biomass under different gradient for planted and bare plot and long-term fertilization treatment. The results show that the range of TOC in planted plot is 49.0~63.9 g·kg^-1 and in bare plot is 47.4~50.8 g·kg^-1, both of which decrease with increasing gradients. The range of MBC in planted plot is 0.9~3.45 g·kg^-1, while in bare plot is only 0.1~0.68 g·kg^-1. The range of TOC under different fertilization treatment is 57.0~64.5 g·kg^-1, and the TOC is significantly higher under application of organic fertilizer than other fertilizer. The TN in planted plot is 2.6~4.2 g·kg^-1 and in bare plot is 1.6~5.5 g·kg^-1. Meanwhile, the MBN is higher in planted plot than in bare plot. The TN under different fertilization treatment significantly decreases with increasing soil depth, and the increase on MBN under organic fertilizer and straw combined with chemical fertilizer is notable. The ratio of C and N under combination of organic and chemical fertilizers is between 10 and 17, under combination of straw and chemical fertilizer is between 8 and 13, and under single application of N is 12. Increase in organic fertilizer and straw return would be helpful for maintaining and enhancing soil C/N. Meanwhile, fertilization treatment would be helpful for increasing microbe entropy and enhancing the proportion of active soil organic C to TOC. The coupling between MBC and TN in the hillslope cropland of purple soil is notable, with R² coefficient of 0.66.

• Key words：C/N  microbial biomass C  microbial biomass N  purple soil

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