卓丽玲,张春岭,张少东,刘福航,张广明,楚文超.钙和铅相互作用对鲫鱼毒性效应的影响[J].环境科学学报,2014,34(8):2175-2178
钙和铅相互作用对鲫鱼毒性效应的影响
- Effect of interaction between calcium and lead on the toxicity of crucian
- 基金项目：枣庄市科技攻关项目（No.201024-1）；山东省自然科学基金（No.ZR2010CL004）
- 摘要：以鲫鱼为材料，研究了钙和铅相互作用对鲫鱼毒性效应的影响.试验分为5组，分别为空白对照Ⅰ组、单独染铅Ⅱ组（0.1 mg·L-1）及铅钙联合组（Ⅲ组：0.1 mg·L-1铅+2 mmol·L-1钙，Ⅳ组：0.1 mg·L-1铅+4 mmol·L-1钙，Ⅴ组：0.1 mg·L-1铅+6 mmol·L-1钙），处理35 d后，检测鲫鱼肌肉、鳃中铅含量，以及肝胰脏中总抗氧化能力（T-AOC）、过氧化氢酶（CAT）活性和丙二醛（MDA）含量.结果表明，与Ⅰ组比较，Ⅱ、Ⅲ组鲫鱼肌肉、鳃中铅含量增高（p<0.05），Ⅴ组鲫鱼肌肉中铅含量降低（p<0.05），与Ⅱ组比较，Ⅳ、Ⅴ组鲫鱼肌肉、鳃中铅含量降低（p<0.05）；与Ⅰ组比较，Ⅱ组CAT活性降低（p<0.05），Ⅲ、Ⅳ和Ⅴ组CAT活性升高（p<0.05），与Ⅱ组比较，Ⅳ、Ⅴ组CAT活性升高（p<0.05）；与Ⅰ组比较，Ⅱ、Ⅲ和Ⅴ组T-AOC活性降低（p<0.05），与Ⅱ组比较，Ⅲ、Ⅳ和Ⅴ组T-AOC活性增高（p<0.05）；与Ⅰ组比较，Ⅱ、Ⅲ、Ⅳ和Ⅴ组MDA含量升高（p<0.05），与Ⅱ组比较，Ⅲ、Ⅳ和Ⅴ组MDA含量降低（p<0.05）.由此可见，钙可竞争鲫鱼对铅的吸收，降低铅在其肌肉、鳃中的蓄积，提高鲫鱼肝胰脏的抗氧化能力，表明钙能降低铅的毒性效应.
- Abstract：This study was to investigate the toxic effects of interaction between calcium (Ca) and lead (Pb) on crucian. It was divided into five groups, i.e., control group (Group Ⅰ), Pb2+ alone exposed group (0.1 mg·L-1, Group Ⅱ), and Pb2+ combined with Ca2+ groups (0.1mg·L-1 Pb2+ + 2 mmol·L-1 Ca2+ (Group Ⅲ), 0.1 mg·L-1 Pb2+ + 4 mmol·L-1 Ca2+ (Group Ⅳ), 0.1 mg·L-1 Pb2+ + 6 mmol·L-1 Ca2+ (Group Ⅴ)). After exposed for 35 days, concentrations of Pb2+ in the muscle and gill and the total anti-Oxidative capacity (T-AOC) as well as levels of catalase (CAT) and malondialdehyde (MDA) in the hepatopancreas were assessed. The results showed that Pb2+ levels in the muscle and gill of Group II and Group III increased (p<0.05) and its level in the muscle of Group V decreased (p<0.05) compared to Group I. Compared with Group II, Pb2+ levels in the muscle and gill of Group IV and Group V decreased significantly (p <0.05). As far as the CAT activity was concerned, its activity in Group II was lower than that in Group I (p <0.05), while its level in other three groups (Group III, Group IV and Group V) was higher than that in Group I (p<0.05), respectively. Compared with Group II, the CAT activity in Group IV and Group V increased significantly (p<0.05). Regarding the T-AOC, its level in three groups (II, III and V) was significantly lower (p<0.05) than that in Group I, but T-AOC level in three groups (III, IV and V) was significantly higher (p<0.05) than that in Group II, respectively. As for MDA, its content in other four groups (II, III, IV and V) was higher (p<0.05) than that in Group I, respectively, while its level in three groups (III, IV and V) was lower (p <0.05) than that in Group II, respectively. In summary, Ca2+ can lower the accumulation of Pb2+ in the muscle and gill of crucian and increase the anti-Oxidative ability in the hepatopancreas by the interaction between Ca2+ and Pb2+, which demonstrated that Ca2+ can alleviate the toxic effect of Pb2+.
摘要点击次数： 1681 全文下载次数： 2285
