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Adhesion characteristics of Chlorella vulgaris cells on solid substrate surface

基金项目：国家自然科学基金重点项目(No.51136007);教育部科学技术研究项目(No.113053A);重庆市自然科学基金重点项目(No.cstc2013jjB9004)

摘要：针对平板式光生物反应器,以补充有碳源的SE(Brostol's solution)培养基作为普通小球藻生长的水环境,研究了载体表面粗糙度、藻细胞接种量、培养液pH值和液体流量对小球藻细胞在固体基质表面吸附特性的影响. 结果表明:藻细胞在各载体表面的吸附密度均随时间进行呈现先快速增大再逐渐趋于平缓的变化趋势;在实验条件下,随载体表面粗糙度和藻液中藻细胞接种量增大,载体表面对小球藻细胞的吸附率均增大,吸附密度也增大;当藻液pH值为6左右时,小球藻在载体表面的吸附密度达到最大;液体流量为0.65 mL·min^-1时,载体表面对小球藻细胞的吸附能力最强. 各参数对藻细胞吸附密度影响的重要程度依次为藻细胞接种量、培养基pH值、液体流量.

Abstract：Adhesion characteristics of Chlorella vulgaris cells on a solid substrate surface were experimentally investigated based on a flat plate photo-bioreactor. SE (Brostol's solution) medium with additional carbon source was used as the liquid growth environment. The effects of surface roughness of substrate, inoculation amount of algal cells, pH value and flow rate of culture medium on the adhesion characteristics of Chlorella vulgaris cells were discussed. The results denoted that the adhesion densities of algal cells on all substrate surfaces increased with time and stabilized gradually. Under the experimental conditions, with the increase of substrate surface roughness and inoculation amount of algal cells in culture medium, both the adhesion rate and adhesion density of Chlorella vulgaris cells were enhanced. Furthermore, it was found that the adhesion performance of Chlorella vulgaris cells on the substrate surface peaked at the pH value of medium of 6 or the liquid flow rate of 0.65 mL·min^-1. The inoculation amount was the most important factor, followed by the pH value of medium and the liquid flow rate.