熊仕娟,徐卫红,杨芸,王崇力,江玲,周坤,刘俊,张明中.不同温度下微生物和纤维素酶对发酵猪粪理化特性的影响[J].环境科学学报,2014,34(12):3158-3165
不同温度下微生物和纤维素酶对发酵猪粪理化特性的影响
- Effects of microbes and cellulase in pig manure fermentation at different temperature
- 基金项目：现代农业产业技术体系建设专项(No. Nycytx-35-gw16);国家科技支撑计划项目(No. 2007BAD87B10)
- 摘要：采用恒温发酵培养试验,研究了5 ℃ 和25 ℃ 时分别接种纤维素酶(X)、枯草芽孢杆菌(K)和EM菌(E)及其组合对新鲜猪粪发酵中的全氮、铵态氮、有机质、pH及微生物数量的影响.结果表明,5 ℃ 条件下,猪粪中微生物生长受抑制,细菌、真菌、放线菌数量均明显低于25 ℃,发酵终期猪粪有机质含量为70%~83%,pH值为7.16~7.36;25 ℃ 条件时,发酵终期猪粪有机质含量降至61%~72%,pH值升至8.09~8.94.与对照相比较,25 ℃ 下添加微生物和纤维素酶的处理有机质含量降低了2.95%~7.70%(除了K和XE处理),C/N值降低了4.04%~37.59%(除了XK处理),pH值增加了1.7%~26.8%.在总的添加量一致的条件下,发酵剂组合KE、XE、XK、XKE对猪粪发酵的效果优于单一发酵剂的X、K、E处理.
- Abstract：The study was conducted to investigate the effects of single or combined treatments of cellulase (X), Bacillus subtilis (K) and effective microorganisms (E) on contents of total N, NH+4-N and organic material, value of T and pH, and microbial quantity during pig manure fermentation at 5 ℃ and 25 ℃, respectively. The results showed that the microbial growth was obviously inhibited at 5 ℃, and the quantities of bacteria, fungi, and actinomycetes were significantly lower than those at 25 ℃. The content of organic matter was 70%~83%, and the pH value is 7.16~7.36 after treatment at 5 ℃. While in the end of culture at 25 ℃, the content of organic matter decreased to 61%~72%, and the pH increased to 8.09~8.94. Compared with the control in which pig manure was not fermented microbes and cellulase, the content of organic matter decreased by 2.95%~7.70%(except K and XE), the value of C/N decreased by 4.04%~37.59%(except XK), and the value of pH increased by 1.7%~26.8% when treated with microbes and cellulase at 25 ℃. The effects of combined treatments with cellulase (X), Bacillus subtilis (K) and effective microorganisms (E) on pig manure fermentation, such as KE, XE, XK or XKE, were superior to the single treatment of X, K or E with the same inoculation amount.
摘要点击次数： 1489 全文下载次数： 3530
