王余,朱雯倩,王娓敏,梁婵娟.微囊藻毒素对水稻幼苗生长与叶绿素荧光的影响[J].环境科学学报,2015,35(2):602-607
微囊藻毒素对水稻幼苗生长与叶绿素荧光的影响
- Effects of microcystins on growth and chlorophyll fluorescence in rice seedlings
- 基金项目：国家大学生创新计划(No.201310295045);国家自然科学基金(No.31370517,31000245)
- 王余
- 江南大学环境与土木工程学院, 无锡 214122
- 朱雯倩
- 江南大学环境与土木工程学院, 无锡 214122
- 王娓敏
- 江南大学环境与土木工程学院, 无锡 214122
- 梁婵娟
- 江南大学环境与土木工程学院, 无锡 214122
- 摘要：为明确被微囊藻毒素(MCs)污染的灌溉水对农业生产的潜在危害,采用水培法研究了不同浓度(1、100、1000、3000 μg·L-1) MCs处理对胁迫期和恢复期内水稻叶片MCs积累量、叶绿素含量和叶绿素荧光参数的影响.结果表明,胁迫处理7 d后,MCs在叶片中的积累量随MCs处理浓度的增大而升高.与CK相比,1 μg·L-1 MCs处理组水稻叶片的各生长指标和叶绿素含量上升,F0下降,Fv/Fm、ETR、qP和qN均未发生显著变化;高浓度(≥100 μg·L-1)处理下,水稻叶片的生长受抑制,叶绿素含量下降,F0上升,Fv/Fm、ETR、qP和qN显著下降.恢复7 d后各处理组水稻叶片MCs的积累量均低于胁迫期,100 μg·L-1 MCs处理组的F0、qN均接近CK,Fv/Fm、ETR和qP虽低于CK却高于胁迫期,表明MCs对光合系统的伤害有一定程度的恢复.1000和3000 μg·L-1MCs处理组的F0依然高于CK,且Fv/Fm、ETR、qP和qN不仅低于CK也低于胁迫期,表明高浓度MCs (≥1000 μg·L-1)降低了水稻叶片PSⅡ原初光能转换效率和PSⅡ潜在活性,对叶片光合功能造成不可逆的伤害.
- Abstract：To evaluate the potential impact of irrigation water contaminated with microcystins (MCs) on agricultural production, we studied the accumulation of MCs, and changes in chlorophyll content and chlorophyll fluorescence parameters in rice seedlings treated with MCs at different concentrations (1, 100, 1000 and 3000 μg·L-1). The results show that MCs accumulation increased with the increase of MCs concentration. MCs at 1 μg·L-1 increased the growth and chlorophyll content in rice leaves whereas ETR, Fv/Fm, qP and qN had no change. High concentrations of MCs (≥100 μg·L-1) decreased the growth, chlorophyll content, Fv/Fm, ETR, qP and qN while increased F0. After a 7-day recovery, MCs accumulation in rice leaves was lower than those measured during the stress period. For the group treated with 100 μg·L-1 MCs, F0 and qN had no obvious change whereas Fv/Fm, ETR and qP were higher than those measured during the stress period although they were still lower than those of the control. The results indicated that the damage caused by MCs on photosynthetic capacity was reversible. When rice seedlings were treated with higher concentrations MCs (1000 and 3000 μg·L-1), Fv/Fm, ETR, qP and qN were still lower than those of the control, even worse than those measured during the exposure period. It was indicated that high concentration MCs reduced the efficiency of primary light energy conversion and the potential activity of PSⅡ. Furthermore, the damage caused by high concentration MCs on photosynthetic function in plants was irreversible.
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