研究论文
李海云,牛世全,孔维宝,达文燕,耿晖,韩彩虹,阎薇如.猪粪堆肥中一株溶磷菌的筛选鉴定及溶磷能力初步测定[J].环境科学学报,2015,35(5):1464-1470
猪粪堆肥中一株溶磷菌的筛选鉴定及溶磷能力初步测定
- Screening and identification of a phosphate solubilizing strain isolated from pig manure compost and determination of its phosphate solubilizing capacity
- 基金项目：国家自然科学基金(No.31260134,30960078)
- 作者
- 单位
- 李海云
- 西北师范大学生命科学学院, 兰州 730070
- 牛世全
- 西北师范大学生命科学学院, 兰州 730070
- 孔维宝
- 西北师范大学生命科学学院, 兰州 730070
- 达文燕
- 西北师范大学生命科学学院, 兰州 730070
- 耿晖
- 西北师范大学生命科学学院, 兰州 730070
- 韩彩虹
- 西北师范大学生命科学学院, 兰州 730070
- 阎薇如
- 西北师范大学生命科学学院, 兰州 730070
- 摘要：从猪粪堆肥中分离纯化得到一株具有较强溶磷能力的菌株PSM-1,进行了形态学和分子生物学鉴定,研究了其溶磷能力和遗传稳定性.结果表明:通过菌落形态和ITS基因序列分析鉴定,菌株PSM-1为产黄青霉(Penicillium chrysogenum);在3种不同无机磷源液体培养基中其溶磷量依次为Ca3(PO4)2(138.36 mg · L-1) >FePO4(117.38 mg · L-1) >AlPO4(113.76 mg · L-1),且PSM-1的溶磷量均与培养液pH值呈现出显著负相关性;以葡萄糖为碳源、硝酸铵为氮源时,菌株PSM-1的溶磷量最高可达195.67 mg · L-1,比初始有效磷高141.42%;该菌株对碳源和氮源的利用效率分别依次为:葡糖糖 >蔗糖 >麦芽糖 >淀粉,硝酸铵 >硝酸钾 >硫酸铵 >草酸铵;经过20次传代培养后PSM-1的溶磷量保持在(124.54±3.50) mg · L-1,说明其溶磷遗传稳定性良好.研究表明:PSM-1菌株具有良好的溶解无机磷的能力,在土壤微生态改良方面具有重要的应用潜力.
- Abstract：A strain PSM-1 with strong ability to dissolve phosphates was isolated from pig manure compost. It was identified by morphology and ITS gene sequence analysis. The phosphate solubilizing ability and genetic stability of PSM-1 were tested. The results indicated that the strain was identified as Penicillium chrysogenum based on its morphological features and ITS gene sequence. The order of dissolving phosphorus in three different inorganic phosphorus liquid media were: Ca3(PO4)2(138.36 mg · L-1)> FePO4(117.38 mg · L-1) >AlPO4(113.76 mg · L-1). Moreover, the quantity of dissolved phosphorus (QDP) and pH values in culture medium had significantly negative correlation. The strain PSM-1 achieved the highest QDP of 195.67 mg · L-1 when glucose as carbon source and ammonium nitrate as nitrogen source, which was higher than the initial available phosphorus of 141.42%. The order of the carbon and source utilization efficiency by the strain was glucose >sucrose> maltose> starch, and ammonium nitrate >nitrate >ammonium sulfate >ammonium oxalate, respectively. The QDP of PSM-1 was maintained around (124.54±3.50) mg · L-1 after 20 times serial subcultivation, which indicated that the strain had a good genetic stability. The results suggested that the strain PSM-1 has desired solubilizing phosphate capacity, and it has potential applications in the soil ecological amendment.
- Key words：pig manure compost phosphate solubilizing strain solubilizing phosphate capacity genetic stability
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