侯保连,李安婕,孙趣.AHLs群体感应信号分子对硝化污泥附着生长及硝化效果的影响[J].环境科学学报,2015,35(9):2773-2779
AHLs群体感应信号分子对硝化污泥附着生长及硝化效果的影响
- The effect of N-acy-homoserine lactones mediated quorum-sensing on the adhesion growth and nitrification of nitrifying sludge
- 基金项目：国家自然科学基金青年基金(No.51208038)
- 侯保连
- 北京师范大学环境学院, 水沙科学教育部重点实验室, 水环境模拟国家重点实验室, 北京 100875
- 李安婕
- 北京师范大学环境学院, 水沙科学教育部重点实验室, 水环境模拟国家重点实验室, 北京 100875
- 孙趣
- 北京师范大学环境学院, 水沙科学教育部重点实验室, 水环境模拟国家重点实验室, 北京 100875
- 摘要：污水生物脱氮系统中的硝化菌生长慢、易流失,人为添加N-酰基高丝氨酸内酯类(AHLs)群体感应信号分子,可能会强化硝化菌生物膜的形成,从而有助于富集硝化菌,提高硝化效率.本研究以采用低碳氮比(C : N=8)人工配置废水驯化100 d后的硝化活性污泥为菌源,人为外加2 μmol · L-1的AHLs信号分子(C8-HSL或OHHL),分析了两种信号分子对硝化污泥静态附着、氨氮动态降解及微生物生长速率的影响.研究结果表明,信号分子OHHL能快速强化微生物附着生长,且能在一定时间内持续发挥作用,有助于硝化生物膜的形成;而信号分子C8-HSL则能明显提高硝化污泥的氨氮降解速率;两种信号分子都能促进硝化污泥生长,提高微生物生长速率,增强硝化污泥活性,加速硝化污泥生物量累积.人为添加C8-HSL或OHHL信号分子,不仅能保证氨氮降解效率还能降低出水硝氮浓度,减轻氮污染.
- Abstract：Nitrifying bacteria grow slowly and are easily lost in biological wastewater treatment system. N-acy-homoserine lactones (AHLs) signal might be used to enhance the formation of nitrifying biofilm, which contributes to the accumulation of nitrifying bacteria and improves the performance of nitrification. In this study, nitrifying activated sludge has been acclimated in the SBR for 100 days with low C:N ratio (8:1) synthetic wastewater. The influence of 2 μmol · L-1 AHLs (C8-HSL or OHHL) on adhesion, ammonia dynamic degradation, and growth rate of nitrifying sludge was investigated. The results indicated that OHHL could strengthen the adhesion growth of nitrifying sludge quickly and maintain effectively in certain period. OHHL could possibly be used to improve formation of nitrifying biofilm. C8-HSL could obviously increase ammonia degradation rate of nitrifying sludge, though it has no positive effect on the sludge adhesion. Both of C8-HSL and OHHL could facilitate nitrifying sludge growth, improve microbial growth rate, enhance microbial activity of nitrifying sludge, and accelerate biomass accumulation. Adding C8-HSL or OHHL could not only achieve good performance of ammonia degradation, but also reduce nitrate concentration in the effluent, which could mitigate the nitrogen pollution of water environment.
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