• 关键词：碳酸酐酶  固定化  生物催化  二氧化碳  吸收

• 基金项目：重庆市自然科学基金重点项目(No.Cstc2013jjB90003);中央高校基本科研业务经费项目(No.CDJZR12140034)

• 唐强
• 重庆大学低品位能源利用技术及系统教育部重点实验室, 重庆 400030

• 朱轶林
• 重庆大学低品位能源利用技术及系统教育部重点实验室, 重庆 400030

• 陈艳容
• 重庆大学低品位能源利用技术及系统教育部重点实验室, 重庆 400030

• 宋赟
• 重庆大学低品位能源利用技术及系统教育部重点实验室, 重庆 400030

• 卞海波
• 重庆大学低品位能源利用技术及系统教育部重点实验室, 重庆 400030

• 摘要：采用海藻酸钠包埋和戊二醛交联法固定碳酸酐酶,比较游离酶和固定化酶的酶学性质,并用固定化酶催化吸收CO₂.实验结果表明:固定化酶的酶活力回收率是56.3%;最适反应温度为30 ℃,最适pH为7,在T=30~40 ℃和pH=6~10区间有稳定和很高的相对酶活力;固定化酶在60 ℃温浴1 h后,能保持39.8%的相对酶活力,而游离酶已经基本完全失去活性,固定化酶在pH=5.0~11.0范围内pH稳定性显著;固定化酶在T=30 ℃下催化吸收CO₂效果显著,10%的反应时间完成了80.2%的催化反应,催化吸收CO₂的量是空白试验的4.2×10³倍,SEM显示反应后的固定化酶表面出现大量形状不规则的小孔,空隙结构变得发达;固定化酶具有良好的储存稳定性和操作稳定性.

• Abstract：The carbonic anhydrase (CA) was embedded with sodium alginate, which was cross-linked with glutaraldehyde. The enzymatic properties of CA were compared between free and immobilized enzyme, and CO₂ catalytic absorption with immobilized CA was studied. The results show that the immobilized CA retains 56.3% of its original activity. The opti-mum pH of immobilized CA is 7, and temperature is 30 ℃. The immobilized CA has high stability and activity at the range of 30~40 ℃ or pH=6~10. The free enzyme lost all its activity when heated at 60 ℃ for 1 h but the immobilized enzyme kept its activity at 39.8%. The pH value of immobilized CA was stable at the range of pH=5.0~11.0. Immobilized enzyme shows significant effect on CO₂ catalytic absorption at 30 ℃. The 80.2% of catalytic reaction took 10% of the reaction time, and catalytic absorption amount of CO₂ is 4.2×10³ times compared to that of the control experiment. The surface of immobilized CA appeared a large number of irregular-shaped holes, and the pore structure becomes rich through scanning electron microscopy (SEM) observation. Furthermore, the immobilized CA enhances enzamatic stability and durability.

• Key words：carbonic anhydrase  immobilization  bio-catalysis  carbon dioxide  absorption

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