• 关键词：1,2,3,4-四氯苯  筛选  降解  氯离子

• 基金项目：国家国际合作专项项目(No.2013DFG50150);国家自然科学基金(No.40971251)

• 杜青平
• 广东工业大学环境科学与工程学院, 广州 510006

• 李斌洲
• 广东工业大学环境科学与工程学院, 广州 510006

• 卢仁钵
• 广东工业大学环境科学与工程学院, 广州 510006

• 李彦旭
• 广东工业大学环境科学与工程学院, 广州 510006

• 许燕滨
• 广东工业大学环境科学与工程学院, 广州 510006

• 张国庆
• 广东工业大学材料与能源学院, 广州 510006

• 摘要：从广东某化学试剂厂周边土壤中筛选、分离并纯化出一株能有效降解1,2,3,4-四氯苯(1,2,3,4-TeCB)的菌株,命名为L-1号菌.探索不同接种量(2%;3%;5%)菌株对25 mg·L^-1的1,2,3,4-四氯苯模拟废水化学需氧量(COD)、氯离子释放浓度、1,2,3,4-四氯苯降解效果影响,并结合气相质谱联用仪(GC-MS)工作站分析菌株对1,2,3,4-TeCB降解产物.结果表明:经16S rDNA鉴定该菌株为枯草芽孢杆菌;菌株接种量为5%对1,2,3,4-TeCB降解效果最佳,在pH=7.0、温度30℃,摇床转速110 r·min^-1条件下反应7d,COD降解率达62.71%;氯离子释放浓度4.98 mg·L^-1;四氯苯降解量达20.47 mg·L^-1.通过对L-1号菌降解1,2,3,4-TeCB产物分析,发现L-1号菌对1,2,3,4-TeCB的降解是通过先开环后脱氯的途径实现的.

• Abstract：A 1,2,3,4-Tetrachlorobenzene (1,2,3,4-TeCB) degrading bacterial strain named L-1 was screened, isolated, and purified from surrounding soil of a chemical reagents factory in Guangdong Province. The degradation characteristics of 1,2,3,4-Tetrachlorobenzene(1,2,3,4-TeCB) in wastewater were studied for the chemical oxygen demand (COD) removal, chlorine release, and degradation rate of 1,2,3,4-TeCB under various strain inoculation (2%, 3% and 5%). The degradation products of 1,2,3,4-TeCB by strain L-1 were analyzed by using gas chromatograph mass spectrometry (GC-MS). The results showed strain L-1 was Bacillus subtilis as identified by partial length of 16S RNA gene sequences. The optimal strain inoculation concentration was 5%. The degradation rate of COD was up to 62.71%, the chlorine released was 4.98 mg·L^-1, and the degradation of 1,2,3,4-TeCB was up to 20.47 mg·L^-1 at the condition of pH 7.0, 30℃, with a rotating speed of 110 r·min^-1 in 7 days. Through the analysis of degradation products of 1,2,3,4-TeCB by GC-MS, it suggests that the degradation pathway of 1,2,3,4-TeCB was benzene ring cleavage firstly, followed by the chlorine removal from the parent compound.

• Key words：1,2,3,4-tetrachlorobenzene  isolation  degradation characteristics  chlorine

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