• 关键词：银纳米颗粒  水葱  早期生长  生理特征

• 基金项目：国家自然科学基金项目(No.41563008,31100420,41001332)

• 解成杰
• 1. 国家高原湿地研究中心, 昆明 650224;2. 西南林业大学, 昆明 650224

• 郭雪莲
• 1. 国家高原湿地研究中心, 昆明 650224;2. 西南林业大学, 昆明 650224

• 郑荣波
• 西南林业大学, 昆明 650224

• 余磊朝
• 1. 国家高原湿地研究中心, 昆明 650224;2. 西南林业大学, 昆明 650224

• 王山峰
• 1. 国家高原湿地研究中心, 昆明 650224;2. 西南林业大学, 昆明 650224

• 摘要：选取挺水植物水葱(Scirpus tabernaemontani)为受试物种,研究柠檬酸钠-银纳米颗粒(N-AgNPs)对水葱早期生长和生理特性的影响.结果表明:N-AgNPs显著促进了水葱叶片的生长,与N-0 mg·L^-1(对照组)相比,N-5 mg·L^-1、N-10 mg·L^-1、N-15 mg·L^-1、N-20 mg·L^-1处理组水葱叶片的生长速率分别增加20.7%、37.6%、42.9%、47.7%,生物量分别增加77.3%、158.2%、95.5%、127%,叶片中叶绿素含量分别增加23.3%、67.7%、85.2%、80.5%.N-AgNPs浓度与水葱的生长速率、生物量、叶绿素的含量呈正相关关系.另一方面,N-AgNPs浓度增大提高了光系统Ⅱ(PSⅡ)反应中心的光能转换效率(F_v/F_m),且N-AgNPs浓度与F_v/F_m呈显著正相关关系.N-5 mg·L^-1处理组水葱叶片PSⅡ功能反应中心开放度(ΔF_v/F_m)最大,光合电子传递速率(ETR)最快,光合电子传递活性(qP)最大.然而,随着N-AgNPs浓度增大,水葱叶片PSⅡ功能反应中心开放度减小,光合电子传递速率逐渐变慢,光合电子传递活性逐渐降低.另外,N-AgNPs浓度增加降低了水葱叶片中的丙二醛含量,膜脂过氧化作用减弱;N-AgNPs浓度增加降低了水葱叶片中脯氨酸含量,水葱自我调节能力减弱.

• Abstract：The effects of Na-Citrate coated silver nanoparticles (N-AgNPs) on early growth and physiological characteristics of Scirpus tabernaemontani were investigated. N-AgNPs significantly promoted the leaf growth rate of Scirpus tabernaemontani. Compared with the N-0 mg·L^-1 group (control group), the leaf growth rates increased by 20.7%, 37.6%, 42.9% and 20.7%, the biomass accumulation increased by 77.3%, 158.2%, 95.5% and 77.3%, and the chlorophyll content in leaves increased by 23.3%, 67.7%, 85.2% and 67.7% in N-5 mg·L^-1, N-10 mg·L^-1, N-15 mg·L^-1, N-20 mg·L^-1 group, respectively. Positive correlations were found between N-AgNPs concentration and leaf growth rate, biomass and chlorophyll content. On the other hand, the PSⅡreaction center of light energy conversion efficiency (F_v/F_m) improved with the increase of N-AgNPs concentration, indicating their significant positive correlation. During the treated groups, PSⅡfunction reaction center of N-5 mg·L^-1 group exhibited a highest opening degree (ΔF_v/F_m reached max), biggest photosynthetic electron transport rate (ETR reached max), and a biggest photosynthetic electron transport activity (qP reached max). However, with the increase of N-AgNPs concentration, PSⅡfunction reaction center opening degree, photosynthetic electron transport rate and photosynthetic electron transport activity gradually decreased. In addition, the malondialdehyde content in leaves decreased with the increase of N-AgNPs concentration, which revealed that N-AgNPs could reduce the membrane lipid peroxidation of scirpus tabernaemontani. The proline content in leaves decreased with the increase of N-AgNPs concentration, which suggested that N-AgNPs could reduce the self-regulation ability of scirpus tabernaemontan.

• Key words：silver nanoparticle  Scirpus tabernaemontani  early growth  physiological characteristics

• 
  摘要点击次数： 1372 全文下载次数： 1827