• 关键词：黄钾铁矾  酸性矿山废水  Fe²⁺氧化率  总Fe沉淀率  矿物形貌

• 基金项目：国家自然科学基金(No.21407102,41371476);山西省自然科学基金(No.2015011022);山西农业大学青年拔尖创新人才项目(No.TYIT201405)

• 张莎莎
• 山西农业大学资源环境学院环境工程实验室, 太谷 030801

• 沈晨
• 山西农业大学资源环境学院环境工程实验室, 太谷 030801

• 刘兰兰
• 山西农业大学资源环境学院环境工程实验室, 太谷 030801

• 崔春红
• 南京农业大学资源与环境科学学院环境工程系, 南京 210095

• 周立祥
• 南京农业大学资源与环境科学学院环境工程系, 南京 210095

• 刘奋武
• 山西农业大学资源环境学院环境工程实验室, 太谷 030801

• 摘要：氧化亚铁硫杆菌(A. ferrooxidans)生物氧化Fe²⁺与石灰中和相耦合是一种具有发展潜力的酸性矿山废水处理工艺.在Fe²⁺生物氧化段提高Fe²⁺氧化与总Fe沉淀效率是调控此类废水高效处理的关键步骤,且该阶段常伴有黄铁矾等次生铁矿物的合成.本研究通过摇瓶实验,在pH 约2.50的K₂SO₄ (8 mmol·L^-1)-FeSO₄ (160 mmol·L^-1)-H₂O酸性硫酸盐体系中按约3×10⁵ cells·mL^-1的浓度接入A. ferrooxidans,在15℃和30℃两个温度水平下,探究附着微生物的黄钾铁矾回流对体系Fe²⁺生物氧化与总Fe沉淀行为的影响.结果表明,15℃条件下培养至144 h,体系pH变化至2.40,Fe²⁺氧化率和总Fe沉淀率分别仅为46.7%和12.2%.当体系接入附着微生物黄钾铁矾10 g·L^-1时,体系Fe²⁺在132 h即可完全氧化.144 h时,体系pH降低至2.24,总铁沉淀率为25.3%.30℃条件下体系Fe²⁺在72 h完全氧化,pH变化至1.89,总Fe沉淀率为34.3%.当体系接入回流的黄钾铁矾10 g·L^-1时,体系Fe²⁺完全氧化时间缩短至60 h,pH降低至1.85,总Fe沉淀率为37.3%.本研究不同处理体系所得次生铁矿物均为黄钾铁矾,附着微生物黄铁矾回流对15℃环境所得黄钾铁矾形貌影响不大,均为粘附紧密、表面光滑的晶体形貌.而30℃环境中,附着微生物黄铁矾回流却使得原本较为分散、晶型棱角明显的黄铁矾晶体结构变得紧密而光滑.本研究结果可为酸性矿山废水处理提供一定的参数支撑.

• Abstract：The combination of Fe²⁺ oxidation by A. ferrooxidans and lime neutralization technology shows a wide application prospect in the acidic mine drainage(AMD) treatment. Improving Fe²⁺ bio-oxidation efficiency and total Fe precipitation are the key steps for AMD treatment. Jarosite, a secondary iron mineral, can be synthesized during Fe²⁺ bio-oxidation process. Enhancement of returned jarosite carring A. ferrooxidans for Fe²⁺ bio-oxidation and total Fe precipitation was investigated at 15℃and 30℃, under the conditions of K₂SO₄(8 mmol·L^-1)-FeSO₄ (160 mmol·L^-1)-H₂O, initial pH ~2.50, A. ferrooxidans inoculation density ~3×10⁵ cells·mL^-1. Results showed that system pH changed from 2.50 to 2.40 in 144 h under 15℃ condition, with a Fe²⁺ bio-oxidation efficiency and a total Fe precipitation efficiency of 46.7% and 12.2%. However, when a 10 g·L^-1 of A. ferrooxidans-absorbed potassium jarosite was added, complete oxidation of Fe²⁺ was achieved at 132 h, with a decreasing pH of 2.24 and a total Fe precipitation efficiency of 25.3% after 144 h. When temperature was elevated to 30℃, a final pH of 1.89, complete oxidation of Fe²⁺ and total Fe precipitation efficiency of 34.3% can be obtained after 72 h of incubation. Similarly, when inoculated with A. ferrooxidans-absorbed potassium jarosite, complete Fe²⁺ oxidation, an even lower pH of 1.85 and higher Fe precipitation efficiency at 37.3% was achieved after 60 h. Potassium jarosite was identified as the main secondary iron mineral phase in all treatments. Under 15℃, bio-synthesized jarosite crystals closely adhered together with smooth surface, and the morphology of jarosite has no obvious change when A. ferrooxidans-adsorbed jarosite was added into systems. However, under 30℃, potassium jarosite crystals dispersed greatly with edge and corners appeared, and the morphology changed to close and smooth when A. ferrooxidans-adsorbed jarosite was added into systems. The outcomes of this study were helpful in the iron-and sulfate-rich acidic water (such as AMD) engineering treatment.

• Key words：potassium jarosite  acidic mine drainage  Fe²⁺ bio-oxidation efficiency  total Fe precipitation efficiency  mineral morphology

• 
  摘要点击次数： 1705 全文下载次数： 2284