• 关键词：Mn²⁺  生物膜  形态结构  降解性能  胞外聚合物(EPS)

• 基金项目：国家自然科学基金（No.51178208，51368024）；云南省教育厅重点项目（No.2013Z123）；昆明理工大学分析测试基金（No.20140503，20150413）

• 陈凯
• 昆明理工大学环境科学与工程学院, 昆明 650500

• 胡学伟
• 昆明理工大学环境科学与工程学院, 昆明 650500

• 赖信可
• 昆明理工大学环境科学与工程学院, 昆明 650500

• 摘要：通过向自制固定床生物膜反应器（FBBR）中投加氯化锰，考察了Mn²⁺对生物膜量、形态结构、降解性能及其胞外聚合物（EPS）组分的影响，并探究了Mn²⁺在生物膜形成中的作用、影响规律及其对生物膜反应器运行效能的影响。结果表明，投加Mn²⁺有助于生物膜形成，增强生物膜致密性，并促进生物膜生长；在生物膜成熟期连续投加10 mg·L^-1 Mn²⁺对一个周期内生物膜的降解活性影响很小，反应器COD和NH⁺₄去除率分别达到85.4%±1.5%和76.3%±1.9%；生物膜不同类型EPS含量的变化表明，Mn²⁺不仅加速了生物膜的成熟和增强了生物膜去除有机物的稳定性，而且还会刺激生物膜分泌产生更多的紧密粘附型EPS，其中的蛋白质和多糖能够保护微生物免受Mn²⁺伤害。

• Abstract：The effects of Mn²⁺ on the biomass, morphological structure, degradation performance and extracellular polymeric substance (EPS) of biofilm were investigated by dosing manganese chloride in the self-made fixed bed biofilm reactors (FBBRs). Meantime, the operational performance of biofilm reactor has also been studied. Results show Mn²⁺ amendment contributed to the biofilm formation, and the compactness of the biofilm structure during the biofilm development. Continuously dosing 10 mg·L^-1 of Mn²⁺ showed little effect on the removal of both organic matter and ammonia nitrogen, which reached 85.4%±1.5% and 76.3%±1.9% respectively in an operating cycle in the biofilm maturation period. Furthermore, with the different types of EPS contents, the Mn²⁺ not only promoted the biofilm maturation also enhanced the stability of the organic matter removal. The stimulated production of TB-EPS, including protein and polysaccharide, both of which can protect microorganisms against the adverse influences caused by Mn²⁺.

• Key words：Mn²⁺  biofilm  morphological structure  degradation performance  EPS

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