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严青云,向垒,吴小莲,姜元能,李彦文,莫测辉,蔡全英,黄献培,赵海明.环丙沙星高、低积累菜心(Brassica campestris L.)抗氧化性能的差异[J].环境科学学报,2016,36(7):2694-2700

Difference of anti-oxidation activities in Chinese flowering cabbages(Brassica campestris L.)with high and low accumulation of ciprofloxacin

基金项目：国家自然科学基金项目（No.41173101，41301337）；中央高校基本科研业务专项基金项目（No.21612103）；广东省高校高层次人才项目；东莞市科技计划项目（No.2012108150003）

摘要：以环丙沙星抗生素（Ciprofloxacin，CIP）高、低积累菜心（油青四九菜心、油绿粗苔菜心）为研究对象，通过水培实验，研究了CIP胁迫下（0~5.0 mg·L^-1），二者抗氧化性能（蛋白质含量、超氧化歧化酶（SOD）活性、过氧化物酶（POD）活性和丙二醛（MDA）含量）的差异。结果表明，不同浓度CIP条件下，高积累菜心根部CIP富集系数（14.6~24.0）大于低积累菜心（11.3~13.5），但二者转运系数相当（0.006~0.014）。同时，高积累菜心根部及地上部的可溶性蛋白质含量、SOD酶活性以及POD酶活性总体大于低积累菜心，而其膜脂过氧化损伤（MDA含量0.29~3.61 nmol·mg^-1）低于后者（MDA含量0.87~6.23 nmol·mg^-1），因此其对CIP耐受能力较强，利于吸收积累CIP。

Abstract：Reponse differences in antioxidant system (protein contents, activities of superoxide dismutase (SOD) and peroxidase (POD) as well as malondialdehyde (MDA) concentrations) between two cultivar of Chinese flowering cabbages with high and low accumulating Ciprofloxacin (CIP) were investigated using hydroponic culture with different CIP concentrations (0~5.0 mg·L^-1). Results showed that CIP bioaccumulation coefficient (14.6~24.0) of the high-CIP accumulator was higher than that of the low-CIP accumulator (11.3~13.5), but transfer coefficients of the two cultivars were compareble. Under CIP stress, the antioxidant system (protein contents, SOD activities, and POD activities) of the high-CIP accumulator were gernally greater than those of the low-CIP accumulator, but its MDA concentrations were lower than that of the low-CIP accumulator (0.29~3.61 nmol·mg^-1). This indicated that the high-CIP accumulator had higher endurance to CIP and thus can accumulate higher CIP than the low-CIP accumulator.