研究报告
罗妮娜.新型消毒副产物碘代乙酸急性暴露诱导斑马鱼肝脏氧化损伤的机理研究[J].环境科学学报,2016,36(8):3088-3094
新型消毒副产物碘代乙酸急性暴露诱导斑马鱼肝脏氧化损伤的机理研究
- Oxidative damage mechanism of Zebrafish (Danio rerio) liver tissue after acute exposure to iodine acetic acid in vivo
- 摘要：具有较好水溶性的碘代乙酸是新型饮用水消毒副产物，其对水生生物的毒效应及机理备受关注.肝脏是鱼类发挥解毒作用的的主要组织，极易受化学污染物影响.本研究以模式水生动物斑马鱼（Danio rerio）为研究对象，采用半静态换水法，研究了碘代乙酸急性暴露诱导鱼体解毒功能组织氧化损伤的毒效应机理.暴露96 h后，相对于对照组，不同剂量碘代乙酸（1μg·L-1、10μg·L-1和100μg·L-1）暴露导致斑马鱼肝脏组织活性氧和丙二醛含量明显增加；超氧化物歧化酶和谷胱甘肽S转移酶活性增强，而过氧化氢酶活性降低；肝脏解毒过程标志性基因CYP1A、CYP3A和GST在mRNA水平表达量不断增加；这些指标的变化都呈现明显的剂量-效应特征.上述研究表明，饮用水消毒过程形成的碘代乙酸对水生生物具有明显的氧化应激效应，鱼体肝脏组织解毒功能基因相对于其它指标更敏感，可作为生物标志物.此外，应当加强饮用水消毒工艺研究，减少碘代乙酸等消毒副产物的形成以降低其对水生生态系统的潜在危害.
- Abstract：With great solubility, iodine acetic acid is a new kind of disinfection by-products in drinking water. Its toxicology and mechanism on aquatic organisms are widely investigated. Liver tissue is the key tissue for fish detoxification to xenobiotics. In this study, oxidative damage mechanism induced by iodine acetic acid on the liver tissue of Zebrafish (Danio rerio) in vivo was demonstrated. After exposure to different concentrations (1 μg·L-1, 10 μg·L-1 and 100 μg·L-1) of iodine acetic acid for 96 h, both ROS and MDA contents significantly increased (p<0.01) compared with control group. The activities of SOD and GST significantly increased, while the activities of CAT decreased. The mRNA expressions of detoxification function genes in the live tissues such as CYP1A, CYP3A and GST significantly increased. All these changes were in dose-dependent manners. These results clearly indicate that iodine acetic acid can induce oxidative stress in aquatic organisms. The detoxification function genes in the live tissues are more sensitive than other bioindicators and they may be acted as useful biomarkers for the evaluation of iodine acetic acid toxicities. For reducing the hazardous impacts, new measures for less formation of iodine acetic acid during the disinfection progress in drinking water plant are urgently needed.
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