• 关键词：盐度  厌氧污泥  胞外聚合物  三维荧光光谱  傅里叶变换红外光谱  污泥容积指数

• 基金项目：国家自然科学基金（No.51178437）；辽宁省教育厅科学技术研究项目（No.L2015028）

• 王子超
• 1. 大连大学环境与化学工程学院, 大连 116622;2. 中国海洋大学环境科学与工程学院, 青岛 266100

• 高孟春
• 中国海洋大学环境科学与工程学院, 青岛 266100

• 魏俊峰
• 大连大学环境与化学工程学院, 大连 116622

• 杨玉锁
• 大连大学环境与化学工程学院, 大连 116622

• 张晶
• 大连大学环境与化学工程学院, 大连 116622

• 于淑萍
• 大连大学环境与化学工程学院, 大连 116622

• 王悦静
• 烟台市环境监测中心站, 烟台 264000

• 摘要：为探讨盐度变化对厌氧污泥胞外聚合物的影响，采用三维荧光（3D-EEM）和傅里叶变换红外（FTIR）技术，研究了不同盐度下升流式厌氧污泥流化床（UASB）中污泥的松散结合型胞外聚合物（LB-EPS）与紧密结合型胞外聚合物（TB-EPS）的变化规律，并分析了LB-EPS和TB-EPS与污泥沉降性间的关系.结果表明，进水盐度由0逐渐增加至8%，厌氧污泥LB-EPS和TB-EPS含量分别由4.94和12.25 mg·g^-1（以VSS计）增加至41.47和43.04 mg·g^-1（以VSS计）；LB-EPS和TB-EPS中蛋白（PN）和多糖（PS）含量的比值分别由3.2和3.8降低至1.8和2.3；污泥容积指数（SVI）与LB-EPS、TB-EPS均正相关；3D-EEM光谱显示，色氨酸蛋白和芳环蛋白荧光峰存在于不同盐度下LB-EPS和TB-EPS中，类胡敏酸荧光峰仅存在于盐度高于4%的TB-EPS中；FTIR分析表明，进水盐度的增加导致LB-EPS和TB-EPS中PS的C-O组分相对含量增加.

• Abstract：The effect of salinity on the loosely bound extracellular polymeric substances (LB-EPS) and tightly bound extracellular polymeric substances (TB-EPS) from anaerobic sludge in an upflow anaerobic sludge blanket (UASB) reactor was investigated using three-dimensional excitation-emission matrix (3D-EEM) fluorescence spectroscopy and Fourier transform infrared (FTIR) spectra. The relationships of the LB-EPS and TB-EPS with sludge volume index (SVI) at different salinities were analyzed. As the increase of influent salinity from 0 to 8%, the LB-EPS and TB-EPS from anaerobic sludge increased from 4.94 and 12.25 mg·g^-1 VSS to 41.47 and 43.04 mg·g^-1 VSS, respectively, and the protein/polysaccharide (PN/PS) ratios in the LB-EPS and TB-EPS decreased from 3.2 and 3.8 to 1.8 and 2.3, respectively. Both the LB-EPS and TB-EPS exhibited positive correlations with SVI. 3D-EEM fluorescence spectroscopy results show that the tryptophan and aromatic protein-like peaks were present in the LB-EPS and TB-EPS at 0~8% salinity, however the humic acid-like peak was only found in the TB-EPS at 4%~8% salinity. Results of FTIR spectrometry exhibit that the increase of influent salinity resulted in the increase of the relative contents of C-O groups of polysaccharide.

• Key words：salinity  anaerobic sludge  extracellular polymeric substances  three-dimensional excitation-emission matrix fluorescence spectroscopy  Fourier-transform infrared spectroscopy  sludge volume index

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